{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:09:25Z","timestamp":1790057365467,"version":"4.0.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,10,1]]},"abstract":"<jats:p>Summary<\/jats:p>\n               <jats:p>A rat parathyroid cell line, with some differentiated properties of the parathyroid gland, synthesizes predominantly a heparan sulphate proteoglycan (HS-PG) typical of cell surface HS-PGs (core protein = \u223c 70 kDa, three to four HS chains of \u223c 30 kDa). A 10 min pulse-chase protocol was used to determine the metabolic fate of the HS-PGs for cells maintained in 2.1 mM-Ca2+ (high Ca) or in 0.05 mM-Ca2+ (low Ca). In low Ca, \u223c 60% of the labelled HS-PGs reach the cell surface (t1\/2= \u223c 15 min) as determined by trypsin accessibility. This population of HS-PGs recycles (t1\/2= \u223c 9 min) between the cell surface and an intracellular (presumably endosome) compartment. After \u223c 2 h, this population of HS-PGs is internalized and rapidly degraded in lysosomes. In high Ca, only \u223c 10% of the HS-PGs reach the cell surface, where they do not recycle. Changing from high to low Ca any time between 30\u2013120 min of chase, rapidly (t1\/2 less than 4 min) redistributes the HS-PGs to the cell surface where they begin recycling; conversely, changing from low to high Ca leads to a rapid sequestration of the cell surface HS-PGs within the cells. Other divalent cations fail to minic the response to Ca2+. The results suggest that most of the HS-PGs in this cell line are anchored in a membrane compartment involved in a transport process between endosomes and the cell surface which is regulated by the concentration of extracellular Ca2+.<\/jats:p>","DOI":"10.1042\/bst0180816","type":"journal-article","created":{"date-parts":[[2015,8,11]],"date-time":"2015-08-11T12:28:05Z","timestamp":1439296085000},"page":"816-818","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Heparan sulphate proteoglycans on rat parathyroid cells recycle in low Ca2+ medium"],"prefix":"10.1042","volume":"18","author":[{"given":"YASUHIRO","family":"TAKEUCHI","sequence":"first","affiliation":[{"name":"Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KAZUSHIGE","family":"SAKAGUCHI","sequence":"additional","affiliation":[{"name":"Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MASAKI","family":"YANAGISHITA","sequence":"additional","affiliation":[{"name":"Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"VINCENT C.","family":"HASCALL","sequence":"additional","affiliation":[{"name":"Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Society Transactions"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemsoctrans\/article-pdf\/18\/5\/816\/521304\/bst0180816.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemsoctrans\/article-pdf\/18\/5\/816\/521304\/bst0180816.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T23:17:12Z","timestamp":1637277432000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemsoctrans\/article\/18\/5\/816\/84166\/Heparan-sulphate-proteoglycans-on-rat-parathyroid"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,10,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1990,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bst0180816","relation":{},"ISSN":["0300-5127","1470-8752"],"issn-type":[{"value":"0300-5127","type":"print"},{"value":"1470-8752","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,10,1]]}}}