{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T16:40:24Z","timestamp":1694623224964},"reference-count":18,"publisher":"Portland Press Ltd.","issue":"6","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,11,1]]},"abstract":"<jats:p>The androgen receptor (AR) is a ligand-activated transcription factor that regulates gene expression in response to the steroids testosterone and dihydrotestosterone. AR-dependent gene expression is likely to play an important role in a number of receptor-associated disorders, such as prostate cancer, spinal bulbar muscular atrophy, male type baldness and hirsutism. The AR contains two transactivation domains, termed AF1 (activation function 1) located in the N-terminus and AF2 (activation function 2) in the C-terminal ligand-binding domain. AF2 exhibits weak transcriptional activity, whereas AF1 is a strong regulator of transcription. Transcriptional regulation by AF1 is thought to be modulated by a number of proteins that interact with this region, and by post-translational modifications. Our focus is on the N-terminal-interacting proteins and their regulation of transcription via interaction with the receptor. To better understand the mechanism of AR-AF1 action, we have reconstituted AR activity in HeLa nuclear extracts using a unique dual reporter gene assay. Multiple LexA-binding sites in the promoter allow transcription to be driven by a recombinant AR-AF1\u2013Lex fusion protein. The findings from initial experiments suggest an increase in transcription initiation and elongation rates by AR-AF1\u2013Lex. The role of protein\u2013protein interactions involving co-activators and basal transcription factors and AR-AF1 activity are discussed.<\/jats:p>","DOI":"10.1042\/bst0321103","type":"journal-article","created":{"date-parts":[[2004,10,28]],"date-time":"2004-10-28T18:00:43Z","timestamp":1098986443000},"page":"1103-1106","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>In vitro<\/i> regulation of reporter gene transcription by the androgen receptor AF1 domain"],"prefix":"10.1042","volume":"32","author":[{"given":"M.A.","family":"Choudhry","sequence":"first","affiliation":[{"name":"School of Medical Sciences, IMS Building, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.J.","family":"McEwan","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, IMS Building, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2004,10,26]]},"reference":[{"key":"2021111719205647600_B1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1677\/erc.0.0110281","volume":"11","author":"McEwan","year":"2004","journal-title":"Endocr. 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