{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:04:42Z","timestamp":1760645082075},"reference-count":26,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,8,1]]},"abstract":"<jats:p>A general strategy for the expression of bacterial membrane transport and receptor genes in Escherichia coli is described. Expression is amplified so that the encoded proteins comprise 5\u201335% of E. coli inner membrane protein. Depending upon their topology, proteins are produced with RGSH6 or a Strep tag at the C-terminus. These enable purification in mg quantities for crystallization and NMR studies. Examples of one nutrient uptake and one multidrug extrusion protein from Helicobacter pylori are described. This strategy is successful for membrane proteins from H. pylori, E. coli, Enterococcus faecalis, Bacillus subtilis, Staphylococcus aureus, Microbacterium liquefaciens, Brucella abortus, Brucella melitensis, Campylobacter jejuni, Neisseria meningitides, Streptomyces coelicolor and Rhodobacter sphaeroides.<\/jats:p>","DOI":"10.1042\/bst0330867","type":"journal-article","created":{"date-parts":[[2005,8,3]],"date-time":"2005-08-03T12:51:58Z","timestamp":1123073518000},"page":"867-872","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Active membrane transport and receptor proteins from bacteria"],"prefix":"10.1042","volume":"33","author":[{"given":"M.","family":"Saidijam","sequence":"first","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.E.","family":"Bettaney","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Szakonyi","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Psakis","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Shibayama","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.L.","family":"Clough","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Blessie","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Abu-bakr","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Baumberg","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Meuller","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.K.","family":"Hoyle","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.L.","family":"Palmer","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Butaye","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, VAR-CODA-CERVA, Groeselenberg 99, B-1180 Ukkel, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Walravens","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, VAR-CODA-CERVA, Groeselenberg 99, B-1180 Ukkel, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.G.","family":"Patching","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"O'Reilly","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.G.","family":"Rutherford","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.M.","family":"Bill","sequence":"additional","affiliation":[{"name":"Aston Academy of Life Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.I.","family":"Roper","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV47AL, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.K.","family":"Phillips-Jones","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.J.F.","family":"Henderson","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2005,8,1]]},"reference":[{"key":"2021111719384724200_B1","first-page":"3","volume-title":"Transporters Factsbook","author":"Henderson","year":"1997"},{"key":"2021111719384724200_B2","doi-asserted-by":"crossref","DOI":"10.1128\/9781555818319","volume-title":"Two-component Signal Transduction","author":"Hoch","year":"1995"},{"key":"2021111719384724200_B3","first-page":"335","volume-title":"Transporter Factsbook","author":"Griffith","year":"1998"},{"key":"2021111719384724200_B4","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/41483","volume":"388","author":"Tomb","year":"1997","journal-title":"Nature (London)"},{"key":"2021111719384724200_B5","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/16495","volume":"397","author":"Alm","year":"1999","journal-title":"Nature (London)"},{"key":"2021111719384724200_B6","doi-asserted-by":"crossref","first-page":"3802","DOI":"10.1073\/pnas.88.9.3802","volume":"88","author":"Seol","year":"1991","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021111719384724200_B7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/MMBR.62.1.1-34.1998","volume":"62","author":"Pao","year":"1998","journal-title":"Microbiol. Biol. Rev."},{"key":"2021111719384724200_B8","unstructured":"Clough J.L. Ph.D. Thesis 2001 Leeds, U.K. University of Leeds"},{"key":"2021111719384724200_B9","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1093\/oso\/9780199637058.003.0006","volume-title":"Membrane Transport \u2013 A Practical Approach","author":"Ward","year":"2000"},{"key":"2021111719384724200_B10","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/S0014-5793(03)01148-7","volume":"555","author":"Saidijam","year":"2003","journal-title":"FEBS Lett."},{"key":"2021111719384724200_B11","unstructured":"Saidijam M. Ph.D. Thesis 2004 Leeds, U.K. University of Leeds"},{"key":"2021111719384724200_B12","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0022-2836(02)00424-2","volume":"320","author":"Potter","year":"2002","journal-title":"J. Mol. Biol."},{"key":"2021111719384724200_B13","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/0378-1119(87)90314-3","volume":"51","author":"Stark","year":"1987","journal-title":"Gene"},{"key":"2021111719384724200_B14","unstructured":"Hoyle C.J. Ph.D. Thesis 2000 Leeds, U.K. University of Leeds"},{"key":"2021111719384724200_B15","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1021\/bi00375a010","volume":"26","author":"Wallace","year":"1987","journal-title":"Biochemistry"},{"key":"2021111719384724200_B16","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1110\/ps.0229603","volume":"12","author":"Wallace","year":"2003","journal-title":"Protein Sci."},{"key":"2021111719384724200_B17","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/S0924-8579(03)00222-X","volume":"22\/3","author":"Morrison","year":"2003","journal-title":"Int. J. Antimicrob. Agents"},{"key":"2021111719384724200_B18","doi-asserted-by":"crossref","first-page":"2377","DOI":"10.1128\/JB.187.7.2377-2385.2005","volume":"187","author":"Liang","year":"2005","journal-title":"J. Bacteriol."},{"key":"2021111719384724200_B19","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1046\/j.1365-2958.2000.02149.x","volume":"38","author":"Yin","year":"2000","journal-title":"Mol. Microbiol."},{"key":"2021111719384724200_B20","first-page":"597","volume":"9","author":"Hirai","year":"2002","journal-title":"Nat. Struct. Biol."},{"key":"2021111719384724200_B21","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1021\/ja038275c","volume":"126","author":"Patching","year":"2004","journal-title":"J. Am. Chem. Soc."},{"key":"2021111719384724200_B22","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1021\/ja037163i","volume":"126","author":"Patching","year":"2004","journal-title":"J. Am. Chem. Soc."},{"key":"2021111719384724200_B23","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1042\/bj3630243","volume":"363","author":"Venter","year":"2002","journal-title":"Biochem. J."},{"key":"2021111719384724200_B24","doi-asserted-by":"crossref","first-page":"4237","DOI":"10.1021\/bi000150m","volume":"39","author":"Le\u00a0Coutre","year":"2000","journal-title":"Biochemistry"},{"key":"2021111719384724200_B25","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1126\/science.1088196","volume":"301","author":"Abramson","year":"2003","journal-title":"Science"},{"key":"2021111719384724200_B26","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1126\/science.1087619","volume":"301","author":"Huang","year":"2003","journal-title":"Science"}],"container-title":["Biochemical Society Transactions"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemsoctrans\/article-pdf\/33\/4\/867\/540374\/bst0330867.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemsoctrans\/article-pdf\/33\/4\/867\/540374\/bst0330867.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,28]],"date-time":"2024-01-28T13:02:18Z","timestamp":1706446938000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemsoctrans\/article\/33\/4\/867\/65109\/Active-membrane-transport-and-receptor-proteins"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,8,1]]},"references-count":26,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2005,8,1]]},"published-print":{"date-parts":[[2005,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bst0330867","relation":{},"ISSN":["0300-5127","1470-8752"],"issn-type":[{"value":"0300-5127","type":"print"},{"value":"1470-8752","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,8,1]]}}}