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A further example, in which the cargo is non-covalently attached to the BioShuttle, is also discussed. Finally, attempts have been made to solve some of the issues surrounding the efficiency of transfer of therapeutic or diagnostic agents and their later activity in the cell.<\/jats:p>","DOI":"10.1042\/bst0350829","type":"journal-article","created":{"date-parts":[[2007,7,24]],"date-time":"2007-07-24T10:34:06Z","timestamp":1185273246000},"page":"829-832","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Generation, application and quantification of clamp\u2013BioShuttle carriers for plasmid delivery into nuclei of prostate cancer cells"],"prefix":"10.1042","volume":"35","author":[{"given":"R.","family":"Pipkorn","sequence":"first","affiliation":[{"name":"Central Section for Peptide Synthesis, German Cancer Research Center, INF 580, D-69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Waldeck","sequence":"additional","affiliation":[{"name":"Division of Biophysics of Macromolecules, German Cancer Research Center, INF 580, D-69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.W.","family":"Jenne","sequence":"additional","affiliation":[{"name":"Clinical Cooperation Unit Radiation Oncology, German Cancer Research Center, INF 280, D-69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Didinger","sequence":"additional","affiliation":[{"name":"Radiation Oncology, University of Heidelberg, INF 400, D-69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Braun","sequence":"additional","affiliation":[{"name":"Division of Molecular Toxicology, German Cancer Research Center, INF 280, D-69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2007,7,20]]},"reference":[{"key":"2021111718583451100_B1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/S1076-6332(03)80738-9","volume":"8","author":"Luker","year":"2001","journal-title":"Acad. 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