{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:17:52Z","timestamp":1790849872431,"version":"4.1.0"},"reference-count":38,"publisher":"Portland Press Ltd.","issue":"5","license":[{"start":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T00:00:00Z","timestamp":1666915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,10,31]]},"abstract":"<jats:p>Recent data from our laboratory has shown that the nucleotide excision repair (NER) proteins UV-damaged DNA-binding protein (UV-DDB), xeroderma pigmentosum group C (XPC), and xeroderma pigmentosum group A (XPA) play important roles in the processing of 8-oxoG. This review first discusses biochemical studies demonstrating how UV-DDB stimulates human 8-oxoG glycosylase (OGG1), MUTYH, and apurinic\/apyrimidinic (AP) endonuclease (APE1) to increase their turnover at damage sites. We further discuss our single-molecule studies showing that UV-DDB associates with these proteins at abasic moieties on DNA damage arrays. Data from cell experiments are then described showing that UV-DDB interacts with OGG1 at sites of 8-oxoG. Finally, since many glycosylases are inhibited from working on damage in the context of chromatin, we present a working model of how UV-DDB may be the first responder to alter the structure of damage containing-nucleosomes to allow access by base excision repair (BER) enzymes.<\/jats:p>","DOI":"10.1042\/bst20220748","type":"journal-article","created":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T08:38:22Z","timestamp":1666946302000},"page":"1481-1488","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The role of UV-DDB in processing 8-oxoguanine during base excision repair"],"prefix":"10.1042","volume":"50","author":[{"given":"Ashna","family":"Nagpal","sequence":"first","affiliation":[{"name":"1UPMC Hillman Cancer Center, University of Pittsburgh, Pittsburgh, PA 15213, U.S.A."},{"name":"2Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sripriya","family":"Raja","sequence":"additional","affiliation":[{"name":"1UPMC Hillman Cancer Center, University of Pittsburgh, Pittsburgh, PA 15213, U.S.A."},{"name":"2Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4009-2478","authenticated-orcid":false,"given":"Bennett","family":"Van Houten","sequence":"additional","affiliation":[{"name":"1UPMC Hillman Cancer Center, University of Pittsburgh, Pittsburgh, PA 15213, U.S.A."},{"name":"2Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2022,10,28]]},"reference":[{"key":"2022103116085083900_BST-2022-0748CC1","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1016\/j.cell.2017.01.002","article-title":"Endogenous DNA damage as a source of genomic instability in cancer","volume":"168","year":"2017","journal-title":"Cell"},{"key":"2022103116085083900_BST-2022-0748CC2","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.freeradbiomed.2017.03.030","article-title":"Oxidative DNA damage & repair: an introduction","volume":"107","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"2022103116085083900_BST-2022-0748CC3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/mutage\/gez048","article-title":"Implications of DNA damage and DNA repair on human diseases","volume":"35","year":"2020","journal-title":"Mutagenesis"},{"key":"2022103116085083900_BST-2022-0748CC4","doi-asserted-by":"publisher","first-page":"2722","DOI":"10.2174\/0929867325666180226110502","article-title":"The clustered DNA lesions - types, pathways of repair and relevance to human health","volume":"25","year":"2018","journal-title":"Curr. Med. Chem."},{"key":"2022103116085083900_BST-2022-0748CC5","doi-asserted-by":"publisher","first-page":"1603","DOI":"10.1016\/j.freeradbiomed.2010.09.006","article-title":"Oxidative stress, inflammation, and cancer: how are they linked?","volume":"49","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"2022103116085083900_BST-2022-0748CC6","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/j.pnpbp.2017.06.036","article-title":"The interplay between inflammation, oxidative stress, DNA damage, DNA repair and mitochondrial dysfunction in depression","volume":"80","year":"2018","journal-title":"Prog. Neuropsychopharmacol. Biol. Psychiatry"},{"key":"2022103116085083900_BST-2022-0748CC7","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1021\/tx0600043","article-title":"Mechanisms of formation, genotoxicity, and mutation of guanine oxidation products","volume":"19","year":"2006","journal-title":"Chem. Res. Toxicol."},{"key":"2022103116085083900_BST-2022-0748CC8","doi-asserted-by":"publisher","first-page":"8449","DOI":"10.1093\/nar\/gks648","article-title":"Involvement of Werner syndrome protein in MUTYH-mediated repair of oxidative DNA damage","volume":"40","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"2022103116085083900_BST-2022-0748CC9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.dnarep.2017.08.010","article-title":"Human OGG1 activity in nucleosomes is facilitated by transient unwrapping of DNA and is influenced by the local histone environment","volume":"59","year":"2017","journal-title":"DNA Repair (Amst.)"},{"key":"2022103116085083900_BST-2022-0748CC10","doi-asserted-by":"publisher","first-page":"3692","DOI":"10.1074\/jbc.RA119.010497","article-title":"The DNA repair enzyme MUTYH potentiates cytotoxicity of the alkylating agent MNNG by interacting with abasic sites","volume":"295","year":"2020","journal-title":"J. Biol. Chem."},{"key":"2022103116085083900_BST-2022-0748CC11","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.mrfmmm.2014.08.002","article-title":"MutY-glycosylase: an overview on mutagenesis and activities beyond the GO system","volume":"769","year":"2014","journal-title":"Mutat. Res."},{"key":"2022103116085083900_BST-2022-0748CC12","doi-asserted-by":"publisher","first-page":"39982","DOI":"10.1074\/jbc.M507854200","article-title":"DDB1-DDB2 (xeroderma pigmentosum group E) protein complex recognizes a cyclobutane pyrimidine dimer, mismatches, apurinic\/apyrimidinic sites, and compound lesions in DNA","volume":"280","year":"2005","journal-title":"J. Biol. Chem."},{"key":"2022103116085083900_BST-2022-0748CC13","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/0027-5107(94)90012-4","article-title":"Xeroderma pigmentosum group E binding factor recognizes a broad spectrum of DNA damage","volume":"310","year":"1994","journal-title":"Mutat. Res."},{"key":"2022103116085083900_BST-2022-0748CC14","doi-asserted-by":"publisher","first-page":"20027","DOI":"10.1074\/jbc.274.28.20027","article-title":"Characterization of DNA recognition by the human UV-damaged DNA-binding protein","volume":"274","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2022103116085083900_BST-2022-0748CC15","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1038\/s41594-019-0261-7","article-title":"Damage sensor role of UV-DDB during base excision repair","volume":"26","year":"2019","journal-title":"Nat. Struct. Mol. Biol."},{"key":"2022103116085083900_BST-2022-0748CC16","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.molcel.2019.04.024","article-title":"Targeted and persistent 8-oxoguanine base damage at telomeres promotes telomere loss and crisis","volume":"75","year":"2019","journal-title":"Mol. Cell"},{"key":"2022103116085083900_BST-2022-0748CC17","doi-asserted-by":"publisher","first-page":"E1862","DOI":"10.1073\/pnas.1323856111","article-title":"Single-molecule analysis reveals human UV-damaged DNA-binding protein (UV-DDB) dimerizes on DNA via multiple kinetic intermediates","volume":"111","year":"2014","journal-title":"Proc. Natl Acad. Sci. U.S.A."},{"key":"2022103116085083900_BST-2022-0748CC18","doi-asserted-by":"publisher","first-page":"102860","DOI":"10.1016\/j.dnarep.2020.102860","article-title":"Expanding molecular roles of UV-DDB: shining light on genome stability and cancer","volume":"94","year":"2020","journal-title":"DNA Repair"},{"key":"2022103116085083900_BST-2022-0748CC19","doi-asserted-by":"publisher","DOI":"10.1590\/1678-4685-gmb-2019-0104","article-title":"Cooperation and interplay between base and nucleotide excision repair pathways: from DNA lesions to proteins","volume":"43","year":"2020","journal-title":"Genet. Mol. Biol."},{"key":"2022103116085083900_BST-2022-0748CC20","doi-asserted-by":"publisher","first-page":"11227","DOI":"10.1093\/nar\/gkaa777","article-title":"The involvement of nucleotide excision repair proteins in the removal of oxidative DNA damage","volume":"48","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"2022103116085083900_BST-2022-0748CC21","doi-asserted-by":"publisher","first-page":"8177","DOI":"10.1093\/nar\/gkab591","article-title":"Single molecule analysis indicates stimulation of MUTYH by UV-DDB through enzyme turnover","volume":"49","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"2022103116085083900_BST-2022-0748CC22","doi-asserted-by":"publisher","first-page":"974","DOI":"10.1038\/s41467-022-28642-9","article-title":"Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins","volume":"13","year":"2022","journal-title":"Nat. Commun."},{"key":"2022103116085083900_BST-2022-0748CC23","doi-asserted-by":"publisher","first-page":"e2100011","DOI":"10.1002\/bies.202100011","article-title":"Chaperones for dancing on chromatin: role of post-translational modifications in dynamic damage detection hand-offs during nucleotide excision repair","volume":"43","year":"2021","journal-title":"Bioessays"},{"key":"2022103116085083900_BST-2022-0748CC24","doi-asserted-by":"publisher","first-page":"1974","DOI":"10.1021\/acs.biochem.7b00017","article-title":"AP-endonuclease 1 accelerates turnover of human 8-oxoguanine DNA glycosylase by preventing retrograde binding to the abasic-site product","volume":"56","year":"2017","journal-title":"Biochemistry"},{"key":"2022103116085083900_BST-2022-0748CC25","doi-asserted-by":"publisher","first-page":"102909","DOI":"10.1016\/j.dnarep.2020.102909","article-title":"Dynamic action of DNA repair proteins as revealed by single molecule techniques: seeing is believing","volume":"93","year":"2020","journal-title":"DNA Repair (Amst.)"},{"key":"2022103116085083900_BST-2022-0748CC26","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.semcdb.2017.06.028","article-title":"Studying protein-DNA interactions using atomic force microscopy","volume":"73","year":"2018","journal-title":"Semin. Cell Dev. Biol."},{"key":"2022103116085083900_BST-2022-0748CC27","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/bs.mie.2017.03.027","article-title":"Single-molecule methods for nucleotide excision repair: building a system to watch repair in real time","volume":"592","year":"2017","journal-title":"Methods Enzymol."},{"key":"2022103116085083900_BST-2022-0748CC28","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.dnarep.2014.02.003","article-title":"Single molecule techniques in DNA repair: a primer","volume":"20","year":"2014","journal-title":"DNA Repair (Amst.)"},{"key":"2022103116085083900_BST-2022-0748CC29","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1038\/nmeth.3735","article-title":"A genetically targetable near-infrared photosensitizer","volume":"13","year":"2016","journal-title":"Nat. Methods"},{"key":"2022103116085083900_BST-2022-0748CC30","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.freeradbiomed.2017.01.029","article-title":"Sunlight damage to cellular DNA: focus on oxidatively generated lesions","volume":"107","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"2022103116085083900_BST-2022-0748CC31","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1038\/nrm3822","article-title":"Understanding nucleotide excision repair and its roles in cancer and ageing","volume":"15","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2022103116085083900_BST-2022-0748CC32","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1007\/s10735-006-9044-7","article-title":"UV-induced ubiquitylation of XPC complex, the UV-DDB-ubiquitin ligase complex, and DNA repair","volume":"37","year":"2006","journal-title":"J. Mol. Histol."},{"key":"2022103116085083900_BST-2022-0748CC33","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/bs.enz.2019.06.004","article-title":"Mechanism and regulation of DNA damage recognition in mammalian nucleotide excision repair","volume":"45","year":"2019","journal-title":"Enzymes"},{"key":"2022103116085083900_BST-2022-0748CC34","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.molcel.2016.08.019","article-title":"Real-time tracking of parental histones reveals their contribution to chromatin integrity following DNA damage","volume":"64","year":"2016","journal-title":"Mol. Cell"},{"key":"2022103116085083900_BST-2022-0748CC35","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1083\/jcb.201106074","article-title":"DDB2 promotes chromatin decondensation at UV-induced DNA damage","volume":"197","year":"2012","journal-title":"J. Cell Biol."},{"key":"2022103116085083900_BST-2022-0748CC36","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1083\/jcb.201112132","article-title":"PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1","volume":"199","year":"2012","journal-title":"J. Cell Biol."},{"key":"2022103116085083900_BST-2022-0748CC37","doi-asserted-by":"publisher","first-page":"10298","DOI":"10.1158\/0008-5472.Can-05-2295","article-title":"Enhanced DDB2 expression protects mice from carcinogenic effects of chronic UV-B irradiation","volume":"65","year":"2005","journal-title":"Cancer Res."},{"key":"2022103116085083900_BST-2022-0748CC38","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1038\/sj.onc.1208211","article-title":"Tumor-prone phenotype of the DDB2-deficient mice","volume":"24","year":"2005","journal-title":"Oncogene"}],"container-title":["Biochemical Society Transactions"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemsoctrans\/article-pdf\/50\/5\/1481\/939037\/bst-2022-0748c.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemsoctrans\/article-pdf\/50\/5\/1481\/939037\/bst-2022-0748c.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T12:09:04Z","timestamp":1667218144000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemsoctrans\/article\/50\/5\/1481\/231996\/The-role-of-UV-DDB-in-processing-8-oxoguanine"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,28]]},"references-count":38,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,10,28]]},"published-print":{"date-parts":[[2022,10,31]]}},"URL":"https:\/\/doi.org\/10.1042\/bst20220748","relation":{},"ISSN":["0300-5127","1470-8752"],"issn-type":[{"value":"0300-5127","type":"print"},{"value":"1470-8752","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,28]]}}}