{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:04:17Z","timestamp":1790661857762,"version":"4.1.0"},"reference-count":84,"publisher":"Portland Press Ltd.","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nanna Therapeutics","award":["NT6A"],"award-info":[{"award-number":["NT6A"]}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We highlight the multifunctionality of the Hel308 and HELQ Ski2-like \u2018helicases\u2019 as they toggle between DNA annealing and DNA translocation across multiple DNA repair pathways. Defects in Hel308\/HELQ cause genetic instability, including dysregulated homologous recombination. New data reveal how Hel308 and HELQ switch between DNA translocation and annealing, a mechanism that may restrain recombination to short tracts by curbing extensive DNA synthesis. We describe a PWI-like innovation in HELQ that evolved in bilaterian animals\u2014absent from other animals and archaea\u2014which we propose is an ATR-targeted phosphoregulatory module, coordinating protein interactions and, through those, DNA repair-recombination reactions. The overall picture is of Hel308 and HELQ as ancient DNA annealing enzymes, aided by short bursts of ATP-powered DNA translocation, kept in balance by molecular switches and the additional regulatory surfaces that arose in some animals.<\/jats:p>","DOI":"10.1042\/bst20250255","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:40:59Z","timestamp":1790660459000},"page":"1375-1385","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["HELQ &amp; Hel308: ancient enzymes of DNA repair and recombination"],"prefix":"10.1042","volume":"54","author":[{"given":"Anna","family":"Lou-Hing","sequence":"first","affiliation":[{"name":"The School of Life Sciences, University of Nottingham, Nottingham, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Downs","sequence":"additional","affiliation":[{"name":"The School of Life Sciences, University of Nottingham, Nottingham, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorsten","family":"Allers","sequence":"additional","affiliation":[{"name":"The School of Life Sciences, University of Nottingham, Nottingham, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5656-7706","authenticated-orcid":false,"given":"Edward\u00a0L.","family":"Bolt","sequence":"additional","affiliation":[{"name":"The School of Life Sciences, University of Nottingham, Nottingham, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"2026092901405035000_B1","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1146\/annurev.biochem.76.052305.115300","article-title":"Structure and mechanism of helicases and nucleic acid translocases","volume":"76","author":"Singleton","year":"2007","journal-title":"Annu. 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