{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T12:17:44Z","timestamp":1777983464108,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":1038,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:p>Adipocyte lines are a useful tool for adipocyte research. Recently, a new preadipocyte line designated AP\u201018 was established from subcutaneous tissue of the C3H\/He mouse. In this study, we further characterized AP\u201018 cells. Adipocyte differentiation was assessed by accumulation of fat droplets stained by Oil Red O. The expression of the preadipocyte\u2010 or adipocyte\u2010specific genes and adipocytokine genes was analysed qualitatively by RT\u2010PCR and quantitatively by real\u2010time PCR in comparison with the LM cell, a murine fibroblast line, and the 3T3\u2010L1 cell, respectively. AP\u201018 cells were fibroblastoid in maintenance culture. After the confluence, fat droplets were accumulated in 50\u201360% of the cells cultured in the medium alone and in 70\u201390% of the cells cultured with insulin within 2 to 3 weeks. The fat accumulation was not promoted by the addition of dexamethazone, IBMX (3\u2010isobutyl\u20101\u2010methylxanthine) or troglitazone in combination with insulin, which were obligatory for differentiation of the 3T3\u2010L1 cell, a murine preadipocyte line. Throughout the differentiation, AP\u201018 cells expressed Pref\u20101, LPL, C\/EBP\u03b2, C\/EBP\u03b4, RXR\u03b1, C\/EBP\u03b1, PPAR\u03b3, RXR\u03b3, aP2, GLUT4, SCD1, UCP2, UCP3, TNF\u03b1, resistin, leptin, adiponectin and PAI\u20101 genes, but not the UCP1 gene, indicating that the cell is derived from WAT (white adipose tissue). The time course of these gene expressions was similar to that of 3T3\u2010L1 cells, although the expressions were slower and lower in AP\u201018 cells. These data indicate that AP\u201018 cells are preadipocytes originated from WAT and differentiate into adipocytes under more physiological conditions than 3T3\u2010L1 cells. AP\u201018 may be useful in adipocyte research.<\/jats:p>","DOI":"10.1042\/cbi20090063","type":"journal-article","created":{"date-parts":[[2009,11,11]],"date-time":"2009-11-11T15:18:18Z","timestamp":1257952698000},"page":"293-299","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Characterization of a novel murine preadipocyte line, AP\u201018, isolated from subcutaneous tissue: Analysis of adipocyte\u2010related gene 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