{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T09:57:27Z","timestamp":1768557447067,"version":"3.49.0"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":916,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:p>As part of a programme of comparative measurements of <jats:italic>P<\/jats:italic> <jats:sub>d<\/jats:sub> (diffusional water permeability) the RBCs (red blood cells) from an aquatic monotreme, platypus (<jats:italic>Ornithorhynchus anatinus<\/jats:italic>), and an aquatic reptile, saltwater crocodile (<jats:italic>Crocodylus porosus<\/jats:italic>) were studied. The mean diameter of platypus RBCs was estimated by light microscopy and found to be \u223c6.3 \u03bcm. <jats:italic>P<\/jats:italic> <jats:sub>d<\/jats:sub> was measured by using an Mn<jats:sup>2+<\/jats:sup>\u2010doping 1H NMR (nuclear magnetic resonance) technique. The <jats:italic>P<\/jats:italic> <jats:sub>d<\/jats:sub> (cm\/s) values were relatively low: \u223c2.1\u00d710<jats:sup>\u22123<\/jats:sup> at 25\u00b0C, 2.5\u00d710<jats:sup>\u22123<\/jats:sup> at 30\u00b0C, 3.4\u00d710<jats:sup>\u22123<\/jats:sup> at 37\u00b0C and 4.5 at 42\u00b0C for the platypus RBCs and \u223c2.8\u00d710<jats:sup>\u22123<\/jats:sup> at 25\u00b0C, 3.2\u00d710<jats:sup>\u22123<\/jats:sup> at 30\u00b0C, 4.5\u00d710<jats:sup>\u22123<\/jats:sup> at 37\u00b0C and 5.7\u00d710<jats:sup>\u22123<\/jats:sup> at 42\u00b0C for the crocodile RBCs. In parallel with the low water permeability, the <jats:italic>E<\/jats:italic> <jats:sub>a,d<\/jats:sub> (activation energy of water diffusion) was relatively high, \u223c35 kJ\/mol. These results suggest that \u201cconventional\u201d WCPs (water channel proteins), or AQPs (aquaporins), are probably absent from the plasma membranes of RBCs from both the platypus and the saltwater crocodile.<\/jats:p>","DOI":"10.1042\/cbi20090430","type":"journal-article","created":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T15:36:44Z","timestamp":1267457804000},"page":"703-708","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comparative NMR studies of diffusional water permeability of red blood cells from different species: XVIII platypus (<i>Ornithorhynchus anatinus)<\/i> and saltwater crocodile (<i>Crocodylus porosus<\/i>)"],"prefix":"10.1002","volume":"34","author":[{"given":"Gheorghe","family":"Benga","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bogdan E.","family":"Chapman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy C.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip W.","family":"Kuchel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"6","article-title":"Who discovered the water channels (aquaporins)?","volume":"52","author":"Balaban AT","year":"2006","journal-title":"Cell Mol Biol"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0079-6107(88)90002-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60864-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1065-6995(03)00171-9"},{"key":"e_1_2_9_6_1","first-page":"3","article-title":"The first water channel protein (later called aquaporin 1) was first discovered in Cluj\u2010Napoca, Romania","volume":"41","author":"Benga Gh","year":"2004","journal-title":"Rom J Physiol"},{"key":"e_1_2_9_7_1","first-page":"99","volume-title":"Proceedings of The 9th World Multi\u2010Conference on Systemics, Cybernetics and Informatics, Orlando, Florida, USA, 2005; Vol.X","author":"Benga Gh"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2302\/kjm.55.64"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4183\/aeb.2006.323"},{"key":"e_1_2_9_10_1","first-page":"10","article-title":"Water channel proteins: from their discovery in 1985 in Cluj\u2010Napoca, Romania, to the 2003 Nobel Prize in Chemistry","volume":"52","author":"Benga Gh","year":"2006","journal-title":"Cell Mol Biol"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.156"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(95)00116-6"},{"key":"e_1_2_9_13_1","first-page":"105","volume-title":"Physiological significance of water channel proteins in the red blood cell membranes: analysis at the level of the cell\u2010 and of the whole\u2010body systems. 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