{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:27:08Z","timestamp":1762079228675},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,9,29]],"date-time":"2014-09-29T00:00:00Z","timestamp":1411948800000},"content-version":"vor","delay-in-days":1581,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:p>\u03b2\u2010Thalassaemia is characterized by a decrease in globin \u03b2\u2010chain synthesis and an excess in free \u03b1\u2010globin chains. This induces alterations in membrane lipids and proteins resulting from a reduction in spectrin\/band 3 ratio, partial oxidation of band 4.1 and clustering of band 3. The membrane injury provokes hyperhaemolysis and bone marrow hyperplasia. The pathophysiology of thalassaemia is associated with iron overload that generates oxygen free radicals and oxidative tissue injury with ocular vessel alterations. The aim of this research is to investigate the influence of oxidative stress on band 3 efficiency, which is an integral membrane protein of RBCs (red blood cells). Band 3 protein, of which there are more than 1 million copies per cell, is the most abundant membrane protein in human RBCs. It mediates the anion exchange and acid\u2013base equilibrium through the RBC membrane. Some experiments were performed on thalassaemic cells and \u03b2\u2010thalassaemia\u2010like cells and tested for sulfate uptake. To test the antioxidant effect of Mg<jats:sup>2+<\/jats:sup>, other experiments were performed using normal and pathological cells in the presence of Mg<jats:sup>2+<\/jats:sup>. The oxidant status in thalassaemic cells was verified by increased K<jats:sup>+<\/jats:sup> efflux, by lower GSH levels and by increased G6PDH (glucose\u20106\u2010phosphate dehydrogenase) activity. The rate constant of SO<jats:sub>4<\/jats:sub> <jats:sup>2\u2212<\/jats:sup> uptake decreases in thalassaemic cells as well as in \u03b2\u2010thalassaemia\u2010like cells when compared with normal cells. It increases when both cells are incubated with Mg<jats:sup>2+<\/jats:sup>. Our data show that oxidative stress plays a relevant role in band 3 function of thalassaemic cells and that antioxidant treatment with Mg<jats:sup>2+<\/jats:sup> could reduce oxidative damage to the RBC membrane and improve the anion transport efficiency regulated by band 3 protein.<\/jats:p>","DOI":"10.1042\/cbi20090472","type":"journal-article","created":{"date-parts":[[2010,5,7]],"date-time":"2010-05-07T15:06:12Z","timestamp":1273244772000},"page":"655-662","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Erythrocytes anion transport and oxidative change in 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