{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:31:13Z","timestamp":1789644673199,"version":"4.0.0"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":551,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Silica is a potent occupational fibrogenic agent capable of inducing lung fibrosis and many other lung diseases. Our current study focused on the signalling pathways regulating cell cycle changes in HELF (human embryo lung fibroblast) after silica (\u03b1\u2010quartz) exposure. Our results showed silica exposure could lead to cell cycle changes. The cell cycle alternations were accompanied with overexpression of cyclin D1 and CDK4 (cyclin\u2010dependent kinase 4) in a time\u2010dependent manner. Silica exposure also decreased E2F\u20104 expression in HELF. These changes were blocked by overexpression of dominant\u2010negative mutants of ERK (extracellular signal\u2010regulated protein kinase) or the JNK (stress\u2010activated c\u2010Jun NH<jats:sub>2<\/jats:sub>\u2010terminal kinase), respectively. Moreover, pretreatment of cells with curcumin, an activation of AP\u20101 (activator protein\u20101) inhibitor, inhibited silica\u2010induced cell cycle alteration, the decreased expression of E2F\u20104 and overexpression of cyclin D1 and CDK4. Furthermore, both antisense cyclin D1 and antisense CDK4 can block silica\u2010induced cell cycle changes. These results suggest that silica exposure can induce cell cycle changes, which may be mediated through ERK, JNK\/AP\u20101\/cyclin D1\u2013CDK4\u2010dependent pathway.<\/jats:p>","DOI":"10.1042\/cbi20100298","type":"journal-article","created":{"date-parts":[[2011,2,22]],"date-time":"2011-02-22T11:05:03Z","timestamp":1298372703000},"page":"697-704","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Roles of the ERK, JNK\/AP\u20101\/cyclin D1\u2013CDK4 pathway in silica\u2010induced cell cycle changes in human embryo lung fibroblast cells"],"prefix":"10.1002","volume":"35","author":[{"given":"Xiaowei","family":"Jia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingci","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianglin","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengmei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-6-129"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0048-9697(00)00781-6"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1999.276.6.L979"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M507951200"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.24.4.4290"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/oem.60.2.122"},{"key":"e_1_2_9_8_1","first-page":"2429","article-title":"Hypoxia activates a platelet\u2010derived growth factor receptor\/phosphatidylinositol 3\u2010kinase\/Akt pathway that results in glycogen synthase kinase\u20103 inactivation","volume":"61","author":"Chen EY","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/oem.55.11.779"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.2.1065"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.12.5282-5291.2005"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/oem.55.4.243"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/67.2.275"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.43.30611"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007666200"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5769(01)00170-9"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1116703"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309763200"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/annhyg\/45.3.201"},{"key":"e_1_2_9_20_1","first-page":"1","volume-title":"IARC Monographs on the evaluation of the carcinogenic risk of chemicals to humans. Silica, some silicates, coal dust and para\u2010aramid fibrils","author":"International Agency for Research on Cancer","year":"1997"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2005.08.014"},{"key":"e_1_2_9_22_1","first-page":"3","article-title":"ERK, JNK\/AP\u20101 pathway was involved in silica\u2010induced cell cycle changes","volume":"26","author":"Jia X","year":"2008","journal-title":"Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/56.2.405"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.010488"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2005.46.5.597"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/23.7.1111"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2003-0284OC"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.8.3324-3337.2005"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i22.3235"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2006-0297OC"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2006-0033OC"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.49.1.18"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00024.2004"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfh214"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00427.2001"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfh110"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015924413043"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1136\/oem.58.1.38"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408039101"},{"key":"e_1_2_9_40_1","first-page":"343","article-title":"Interleukin\u20101alpha enhances IL\u20108 secretion through p38 mitogen\u2010activated protein kinase and reactive oxygen species signaling in human pancreatic cancer cells","volume":"11","author":"Sawai H","year":"2005","journal-title":"Med Sci Monit"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.24.4.4181"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2005.07.009"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2008.07.015"},{"key":"e_1_2_9_44_1","first-page":"10","article-title":"Reactive oxygen species and caspase activation mediate silica\u2010induced apoptosis in alveolar macrophages","volume":"280","author":"Shen HM","year":"2001","journal-title":"Am J Respir Cell Mol Biol."},{"key":"e_1_2_9_45_1","first-page":"1791","article-title":"Silica\u2010induced activation of c\u2010Jun\u2010NH2\u2010terminal amino kinases, protracted expression of the activator protein\u20101 proto\u2010oncogene, fra\u20101, and S\u2010phase alterations are mediated via oxidative stress","volume":"61","author":"Shukla A","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.390506"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.14.511"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/12.2.139"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1042%2FCBI20100298","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1042\/CBI20100298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T16:52:39Z","timestamp":1696697559000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1042\/CBI20100298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7]]},"references-count":47,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["10.1042\/CBI20100298"],"URL":"https:\/\/doi.org\/10.1042\/cbi20100298","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}