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The present study investigated the effect of thiol\u2010supplemented medium on cell growth, phenotype and total GSH of cultured hPMCs (human peritoneal mesothelial cells). Cells were cultured in medium M199 supplemented with 2% serum, with \u2018plus\u2019 or without \u2018minus\u2019 <jats:sc>l<\/jats:sc>\u2010Cys and compared with medium supplemented with either \u03b2\u2010ME (\u03b2\u2010mercaptoethanol) (0.25 mmol\/l) or the receptor tyrosine kinase ligand EGF (epidermal growth factor, 100 ng\/ml). \u03b2\u2010ME produced a disproportionate increase in total GSH compared with <jats:sc>l<\/jats:sc>\u2010Cys and other thiols tested [(procysteine (2\u2010oxothiazolidine\u20104\u2010carboxylic acid) or NAC (<jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine)], while growth and morphology were identical. Cell behaviour of primary hPMCs is characterized by the transition of fibroblastoid to cobblestone morphology during early passage. <jats:sc>l<\/jats:sc>\u2010Cys and \u03b2\u2010ME promoted a rapid MET (mesenchymal\u2010to\u2010epithelial transition) within 3 days of culture, confirmed by the presence of cobblestone cells, intact organelles, abundant microvilli, primary cilia and cortical actin. In contrast, EGF produced a biphasic response consisting of delayed growth and retention of a fibroblastoid morphology. During a rapid log phase of growth, MET was accompanied by rapid catch\u2010up growth. Thiols may stabilize the epithelial phenotype by engaging redox\u2010sensitive receptors and transcription factors that modulate differentiation. These data may benefit researchers working on thiol\u2010mediated cell differentiation and strategies to regenerate damage to serosal membranes.<\/jats:p>","DOI":"10.1042\/cbi20100593","type":"journal-article","created":{"date-parts":[[2011,3,17]],"date-time":"2011-03-17T12:05:26Z","timestamp":1300363526000},"page":"857-867","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Thiols stabilize cobblestone morphology of cultured mesothelial cells"],"prefix":"10.1002","volume":"35","author":[{"given":"Stephen D","family":"Bird","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Legge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert 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