{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:39:28Z","timestamp":1791257968047,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":459,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>ADAMs (a disintegrin and metalloproteinase) are a family of type I transmembrane glycoproteins related to snake venom metalloproteases and disintegrins. They are regulatory proteins that modulate intercellular adhesion and the bioavailability of growth factors, and have been implicated in many disease states, including cancer, immunity and inflammation. One member of the ADAM family, ADAM28, has been reported to bind to the integrin \u03b14\u03b21 in humans; however, the distribution of ADAM28 and the biological consequences of ADAM28\u2013\u03b14\u03b21 interactions are yet to be fully elucidated. The expression of ADAM28 in human and murine tissues was examined by multiple Affymetrix microarray analyses, real\u2010time RT\u2014PCR (reverse transcription\u2014PCR) and immunohistochemical staining. We found that ADAM28 has a relatively restricted expression pattern in mouse and human and is highly expressed in the B\u2010lymphocyte lineage, including chronic lymphocytic leukaemic B\u2010cells. The murine B\u2010lymphoma line L1\u20102 and recombinant soluble murine ADAM28 were used to investigate ADAM28\u2013\u03b14\u03b21 interactions. Our data reveal that ADAM28 binding to \u03b14\u03b21 is typical of integrin\u2014ligand interactions, since it is attenuated by anti\u2010functional integrin antibodies, and is enhanced by Mn<jats:sup>2+<\/jats:sup> and the integrin mAb (monoclonal antibody) 9EG7. However, a key finding was that soluble ADAM28 unexpectedly enhanced \u03b14\u03b21\u2010dependent cell adhesion to VCAM\u20101 (vascular cell adhesion molecule\u20101). In so doing ADAM28 was able to influence lymphocyte adhesion to, and migration through, endothelial monolayers, suggesting a physiological role for ADAM28 in regulating the specific spatial and temporal transendothelial migration of lymphocytes.<\/jats:p>","DOI":"10.1042\/cbi20100885","type":"journal-article","created":{"date-parts":[[2011,2,22]],"date-time":"2011-02-22T11:03:53Z","timestamp":1298372633000},"page":"1043-1053","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Modulation of integrin \u03b14\u03b21 by ADAM28 promotes lymphocyte adhesion and transendothelial migration"],"prefix":"10.1002","volume":"35","author":[{"given":"Owen J","family":"McGinn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William R.","family":"English","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Roberts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Ager","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Newham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.128.6.1243"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.2.345"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.153.9.3847"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.4049\/jimmunol.155.4.1671","article-title":"TABS, a T cell activation antigen that induces LFA\u20101\u2010dependent aggregation","volume":"155","author":"Andrew DP","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.4941"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.111.9.1165"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.43.25570"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(98)80042-2"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80519-X"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109538200"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi026871y"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20041444"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi702447u"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600944"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103194200"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)80354-W"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2008.08.001"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90661-W"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(01)00871-X"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3480021"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02506-6"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03098"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90115-S"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803388"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117650"},{"key":"e_1_2_9_27_1","doi-asserted-by":"crossref","first-page":"938","DOI":"10.4049\/jimmunol.155.2.938","article-title":"Vascular cell adhesion molecule\u2014Ig fusion protein selectively targets activated alpha 4\u2010integrin receptors in vivo. 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