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However, whether Pfn, a main secretory vesicle content, indispensible to granzyme B killing, undergoes endocytosis remains unknown. We demonstrate that Pfn is recaptured by early endosomes of NK cells via a clathrin\u2010dependent endocytosis after target cell stimulation. Inhibition of clathrin\u2010dependent endocytosis significantly attenuated the cytotoxicity of NK cells. The data suggest that the recovery of Pfn contributes to the cytotoxicity of NK cells. The assay of endocytosis of lytic molecule presents a particular focus for exploring the mechanism of abnormal cytotoxicity of NK cells.<\/jats:p>","DOI":"10.1042\/cbi20110242","type":"journal-article","created":{"date-parts":[[2011,10,11]],"date-time":"2011-10-11T08:34:11Z","timestamp":1318322051000},"page":"223-228","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Perforin is recaptured by natural killer cells following target cells stimulation for 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