{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T08:21:42Z","timestamp":1715761302546},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":123,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>B6.Y<jats:sup>Tir<\/jats:sup> (mice with Y chromosome from a strain in Tirano, Italy, and autosomes and X\u2010chromosomes from the B6 strain) mice provide an excellent model for analysing sex development that occurs during gonadal differentiation; however, the molecular mechanisms that contribute to sex reversal are unclear. Our aim has been to establish which molecular events participate in this sex reversal. The pattern of gene expression related to testicular [Sry (sex\u2010determining region of the Y chromosome), Sox9 (Sry\u2010related high\u2010mobility group box gene 9) and Mis (M\u00fcllerian\u2010inhibiting substance)] and ovarian [Wnt4 (Wingless\u2010type MMTV (murine\u2010mammary\u2010tumour virus) integration site family, member 4), Rspo1 (cysteine\u2010rich secretory protein containing a thrombospondin type 1 repeat) and Stra8 (stimulated by retinoic acid gene 8)] differentiation was analysed by applying immunofluorescence and real\u2010time RT\u2014PCR (reverse transcription\u2014PCR), focusing on XY gonads from the B6.Y<jats:sup>Tir<\/jats:sup> mouse, but also analysing the normal strains CD\u20101 and C57BL\/6J (B6). The expression of genes related to the process of sexual differentiation was altered in the case of the B6.Y<jats:sup>Tir<\/jats:sup> strain, both at the transcript and protein level, inducing differentiation of ovaries and ovotestes, but not the formation of the testes, which were normal. Our results indicate that the expression of testicular genes is inhibited at various levels, permitting the expression of ovarian genes such as Wnt4, Stra8 and Rspo1. However, their activity was not clear when the data were averaged. Correlation analysis indicated that an ovary differentiation pathway is activated when the testicular differentiation pathway is inhibited.<\/jats:p>","DOI":"10.1042\/cbi20110653","type":"journal-article","created":{"date-parts":[[2012,5,16]],"date-time":"2012-05-16T09:02:34Z","timestamp":1337158954000},"page":"811-822","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Different levels of testicular organization during gonadal differentiation in B6.Y<sup>Tir<\/sup> mice manifesting sex reversal"],"prefix":"10.1002","volume":"36","author":[{"given":"Nivia Rocio","family":"Antonio\u2010Rubio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norma","family":"Moreno\u2010Mendoza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0807297105"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1919"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/348448a0"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.modgep.2004.05.001"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.079822"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.11.030"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn016"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2007-0250"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7089579"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21183"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.135.1.262"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.6.1603"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.029587"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)36689-2"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.1.2305"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040187"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/351117a0"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510813103"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1673-8527(08)60107-1"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.1026"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1994.1266"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8584"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn193"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.1020150506"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.025692"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.016824"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq027"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.modgep.2006.08.006"},{"key":"e_1_2_10_31_1","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1242\/dev.113.2.709","article-title":"The Mus musculus domesticus Tdy allele acts later than the Mus musculus musculus Tdy allele: a basis for XY sex\u2010reversal in C57BL\/6\u2010YPOS mice","volume":"113","author":"Palmer SJ","year":"1991","journal-title":"Development"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2011.04.007"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.069492"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1907"},{"key":"e_1_2_10_35_1","first-page":"72","article-title":"Cloning and expression of R\u2010Spondin1 in different vertebrates suggests a conserved role in ovarian development","volume":"8","author":"Smith CA","year":"2008","journal-title":"Dev Biol."},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(91)90240-4"},{"key":"e_1_2_10_37_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1242\/dev.107.1.95","article-title":"Development and fertility of ovaries in the B6.YDOM sex\u2010reversed female mouse","volume":"197","author":"Taketo\u2010Hosotani T","year":"1989","journal-title":"Development"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20665"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016366"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn036"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(00)00283-5"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/17068"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.10.034"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2009.02.006"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1042%2FCBI20110653","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1042\/CBI20110653","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T16:28:48Z","timestamp":1698769728000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1042\/CBI20110653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9]]},"references-count":43,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1042\/CBI20110653"],"URL":"https:\/\/doi.org\/10.1042\/cbi20110653","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}