{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T07:03:54Z","timestamp":1769756634962,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":62,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Autophagy is usually up\u2010regulated to provide more ATP in response to starvation or OGD (oxygen\u2010glucose deprivation), but the relationship between autophagy and ATP, [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> (intracellular free Ca<jats:sup>2+<\/jats:sup> concentration) or MMP (mitochondrial membrane potential) during reoxygenation is not yet fully clear. The role of autophagy is unknown in PC12 cells subjected to 2 h OGD with different time points of reoxygenation. In the present study, we showed that Beclin\u20101 was up\u2010regulated beginning at 0 h reoxygenation peaking at 24 h and lasting for 48 h. Cell viability was decreased from 0 to 48 h reoxygenation, reaching its minimum at 10 h reoxygenation. ATP was decreased from 0 to 10 h reoxygenation, reaching its minimum at 4 h reoxygenation. A significant negative correlation was observed between ATP and Beclin\u20101 (<jats:italic>r<\/jats:italic>\u200a=\u200a\u22120.61, <jats:italic>P<\/jats:italic>&lt;0.05) at 0 h reoxygenation, but ATP was not significant related (<jats:italic>r<\/jats:italic>\u200a=\u200a0.24, <jats:italic>P<\/jats:italic>&gt;0.05) to Beclin\u20101 at 24 h reoxygenation. Besides, Nimodipine, a calcium antagonist, significantly reduced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and Beclin\u20101, but increased MMP in OGD\/R\u2010treated cells. At 24 h reoxygenation, Beclin\u20101 expression reached its maximum, cell viability continued to increase, and ATP was higher than that before OGD. These results suggest that energy metabolism dysfunction can induce autophagy during OGD in PC12 cells. Increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and decreased MMP may induce autophagy during reoxygenation in PC12 cells. Autophagy may be a protective effect on PC12 cells treated with different time points of reoxygenation after 2 h OGD.<\/jats:p>","DOI":"10.1042\/cbi20120229","type":"journal-article","created":{"date-parts":[[2012,8,28]],"date-time":"2012-08-28T09:36:17Z","timestamp":1346146577000},"page":"1043-1048","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Change of Beclin\u20101 dependent on ATP, [Ca<sup>2+<\/sup>]<sub>i<\/sub> and MMP in PC12 cells following oxygen\u2010glucose deprivation\u2010reoxygenation injury"],"prefix":"10.1002","volume":"36","author":[{"given":"Zhentao","family":"Mo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongqi","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuping","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuehong","family":"Ke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.6.6.12376"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.05849.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20071427"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.jnen.0000229233.75253.be"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.6.7.12753"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-011-9720-3"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.090463"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.7.2424"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2010.01.015"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.203703"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/45257"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2012.01.016"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2010.12.008"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200910140"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000261924.76669.36"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2012.35"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20740"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-008-0117-3"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.5924"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.5598"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-16-19"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2009.12.013"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1042%2FCBI20120229","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1042\/CBI20120229","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T19:49:46Z","timestamp":1698781786000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1042\/CBI20120229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11]]},"references-count":22,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1042\/CBI20120229"],"URL":"https:\/\/doi.org\/10.1042\/cbi20120229","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}