{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T07:51:51Z","timestamp":1698911511569},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1971,3,1]]},"abstract":"<jats:p>1. A vacuum skin-blistering technique has been successfully applied and the human epidermal tissue so obtained has been examined for glycogen content and some of the enzymes involved in glycogen metabolism.<\/jats:p>\n               <jats:p>2. Normal values for glycogen phosphorylase, acid \u03b1-glucosidase and amylo-1,6-glucosidase (debranching enzyme) in epidermis are reported. Glucose 6-phosphatase activity was not detected.<\/jats:p>\n               <jats:p>3. Examination of two patients with Type II glycogen storage disease (Pompe's Disease\u2014lack of lysosomal acid \u03b1-glucosidase) revealed an absence of the acid \u03b1-glucosidase in their skin.<\/jats:p>\n               <jats:p>4. The enzymic lesion in Type V glycogen storage disease (McArdle's Disease\u2014lack of muscle phosphorylase) was not reflected in the epidermal tissue of a patient and a normal level of the enzyme was observed.<\/jats:p>","DOI":"10.1042\/cs0400261","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:25:02Z","timestamp":1438943102000},"page":"261-269","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Enzymes of Glycogen Metabolism in Human Skin with Particular Reference to Differential Diagnosis of the Glycogen Storage Diseases"],"prefix":"10.1042","volume":"40","author":[{"given":"P. D.","family":"Leathwood","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Royal Free Hospital School of Medicine, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brenda E.","family":"Ryman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Royal Free Hospital School of Medicine, London"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/40\/3\/261\/814047\/cs0400261.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/40\/3\/261\/814047\/cs0400261.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T12:42:37Z","timestamp":1638448957000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/40\/3\/261\/69734\/Enzymes-of-Glycogen-Metabolism-in-Human-Skin-with"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1971,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0400261","relation":{},"ISSN":["0009-9287"],"issn-type":[{"value":"0009-9287","type":"print"}],"subject":[],"published":{"date-parts":[[1971,3,1]]}}}