{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,8]],"date-time":"2024-09-08T06:24:34Z","timestamp":1725776674865},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1973,8,1]]},"abstract":"<jats:p>1. A monolayer cell culture was established from a hypernephroma removed from a patient with the diagnosis of ectopic parathyroid hormone (PTH) production.<\/jats:p>\n               <jats:p>2. Culture media contained immunoreactive peptides of a similar size to PTH and several of smaller size.<\/jats:p>\n               <jats:p>3. [14C]Leucine and 75Se-labelled methionine added to the media were incorporated into those peptides in the cells and medium which behaved as PTH immunologically and physicochemically.<\/jats:p>\n               <jats:p>4. A basic peptide resembling proPTH (the biosynthetic precursor of PTH) was synthesized.<\/jats:p>\n               <jats:p>5. Labelled immunoreactive fragments of PTH were found in cells after incubation with radioactive amino acids.<\/jats:p>","DOI":"10.1042\/cs0450183","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:26:23Z","timestamp":1438943183000},"page":"183-191","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Synthesis and Release of Parathyroid Hormone by a Renal Carcinoma in Cell Culture"],"prefix":"10.1042","volume":"45","author":[{"given":"P. B.","family":"Greenberg","sequence":"first","affiliation":[{"name":"1University of Melbourne, Department of Medicine, Austin Hospital, Heildelberg, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. J.","family":"Martin","sequence":"additional","affiliation":[{"name":"2University of Melbourne, Department of Medicine, Austin Hospital, Heildelberg, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen S.","family":"Sutcliffe","sequence":"additional","affiliation":[{"name":"3University of Melbourne, Department of Medicine, Austin Hospital, Heildelberg, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/45\/2\/183\/738533\/cs0450183.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/45\/2\/183\/738533\/cs0450183.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T06:10:03Z","timestamp":1571983803000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/45\/2\/183\/69730\/Synthesis-and-Release-of-Parathyroid-Hormone-by-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1973,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0450183","relation":{},"ISSN":["0301-0538"],"issn-type":[{"value":"0301-0538","type":"print"}],"subject":[],"published":{"date-parts":[[1973,8,1]]}}}