{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T21:15:13Z","timestamp":1649020513972},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1975,10,1]]},"abstract":"<jats:p>1. The pathogenesis of the mental retardation in phenylketonuria remains obscure. Leucocytes have proved of value in the study of other inborn errors of metabolism. The lymphocyte is a suitable model cell for the study of mammalian metabolism, because of its ability to divide in vitro in response to various stimuli.<\/jats:p>\n               <jats:p>2. We have examined the effects of phenylalanine, phenylpyruvate, phenyl-lactate and phenylacetate on the human leucocyte and the resting and phyto-haemagglutinin-stimulated rabbit lymphocyte.<\/jats:p>\n               <jats:p>3. Phenylpyruvate and phenyl-lactate reduced acetate incorporation into leucocyte lipid by 38% and 48% respectively. Only phenyl-lactate reduced acetate incorporation into the resting and stimulated lymphocyte, by 20% and 34% respectively.<\/jats:p>\n               <jats:p>4. Glucose incorporation into leucocyte lipid was unaffected by phenylalanine, phenylpyruvate and phenyl-lactate. Only phenyl-lactate inhibited (46%) the production of CO2 from glucose.<\/jats:p>\n               <jats:p>5. Phenylalanine and leucine incorporation into trichloroacetic acid-insoluble material of resting and stimulated lymphocytes was inhibited by phenyl-lactate (10\u201342%), phenylpyruvate (27\u201357%) and phenylacetate (19\u201339%).<\/jats:p>\n               <jats:p>6. Uridine incorporation into resting and stimulated cells was inhibited by phenyl-lactate (22\u201326%), phenylpyruvate (42\u201352%) and phenylacetate (20%).<\/jats:p>\n               <jats:p>7. Thymidine incorporation into resting lymphocytes was reduced by phenyl-lactate, phenylpyruvate, phenylacetate and phenylalanine by 12\u201326%. Incorporation into the stimulated cell was inhibited by phenylpyruvate and phenyl-lactate (90%) and phenylacetate (66%).<\/jats:p>\n               <jats:p>8. Phenylalanine inhibited lymphocyte pyruvate kinase and phenylpyruvate inhibited citrate synthetase.<\/jats:p>\n               <jats:p>9. These results are compared with published data relating to experimental hyperphenylalaninaemia and the effects of these metabolites on nervous tissue in vitro.<\/jats:p>","DOI":"10.1042\/cs0490343","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T06:29:47Z","timestamp":1438928987000},"page":"343-351","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Phenylalanine and Its Metabolites on the Metabolism of Leucocytes and Lymphocytes"],"prefix":"10.1042","volume":"49","author":[{"given":"D. M.","family":"Bradley","sequence":"first","affiliation":[{"name":"Department of Medicine, The Welsh National School of Medicine, Cardiff, Wales"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. F.","family":"Mahler","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Welsh National School of Medicine, Cardiff, Wales"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/49\/4\/343\/738944\/cs0490343.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/49\/4\/343\/738944\/cs0490343.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,27]],"date-time":"2019-10-27T04:57:08Z","timestamp":1572152228000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/49\/4\/343\/70279\/Effect-of-Phenylalanine-and-Its-Metabolites-on-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1975,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0490343","relation":{},"ISSN":["0301-0538"],"issn-type":[{"value":"0301-0538","type":"print"}],"subject":[],"published":{"date-parts":[[1975,10,1]]}}}