{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,5,14]],"date-time":"2023-05-14T21:25:20Z","timestamp":1684099520447},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1976,5,1]]},"abstract":"<jats:p>1. The combination of a wire-mesh support with the roller-tube technique is described as a procedure for the culture of human jejunal mucosa in vitro.<\/jats:p>\n               <jats:p>2. The technique has been applied to fragments (approximately 10 mg) of jejunal biopsies from both normal subjects and patients with coeliac disease.<\/jats:p>\n               <jats:p>3. The cultured tissue has been shown by radioautography to incorporate [3H]leucine into proteins of the villus epithelial cells and [3H]thymidine into nucleic acid, predominantly by the enterobiasis.<\/jats:p>\n               <jats:p>4. Although the tissue protein and DNA contents fall during culture, it was found that the combined tissue and medium DNA content remained constant during culture and may be used as a reference for enzyme and biochemical studies on cultured intestinal biopsies.<\/jats:p>","DOI":"10.1042\/cs0500425","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:30:40Z","timestamp":1438943440000},"page":"425-429","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biochemical and Morphological Studies on Human Jejunal Mucosa Maintained in Culture"],"prefix":"10.1042","volume":"50","author":[{"given":"C.","family":"L'hirondel","sequence":"first","affiliation":[{"name":"Royal Postgraduate Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. F.","family":"Doe","sequence":"additional","affiliation":[{"name":"Royal Postgraduate Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. J.","family":"Peters","sequence":"additional","affiliation":[{"name":"Royal Postgraduate Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/50\/5\/425\/816452\/cs0500425.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/50\/5\/425\/816452\/cs0500425.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,26]],"date-time":"2019-10-26T10:28:03Z","timestamp":1572085683000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/50\/5\/425\/70531\/Biochemical-and-Morphological-Studies-on-Human"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1976,5,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0500425","relation":{},"ISSN":["0301-0538"],"issn-type":[{"value":"0301-0538","type":"print"}],"subject":[],"published":{"date-parts":[[1976,5,1]]}}}