{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:46:09Z","timestamp":1756993569298},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1978,8,1]]},"abstract":"<jats:p>1. The effects of varying Pco2 on glucose output and the intracellular concentrations of lactate, pyruvate, phosphoenolpyruvate, 2-phosphoglycerate and 3-phosphoglycerate were studied in the isolated rat liver perfused with differing concentrations of lactate.<\/jats:p>\n               <jats:p>2. When the perfusate lactate concentration is above 1.5 mmol\/l respiratory acidosis (simulated by high perfusate Pco2) inhibits gluconeogenesis from lactate, whereas respiratory alkalosis stimulates gluconeogenesis.<\/jats:p>\n               <jats:p>3. In general there were significant positive correlations between intracellular pH (pHl) and hepatocyte phosphoenolpyruvate, 2-phosphoglycerate and 3-phosphoglycerate concentrations, and negative correlations between pHl and lactate and pyruvate concentrations; there were usually significant correlations in the opposite sense between these metabolites and log Pco2.<\/jats:p>\n               <jats:p>4. The results suggest that CO2 exerts an inhibitory effect on gluconeogenesis at a step between pyruvate and phosphoenolpyruvate; however, this is not the only effect of CO2 on the gluconeogenic sequence. CO2 probably acts by changing pHl, but direct effects of CO2 and HCO\u22123 cannot be excluded.<\/jats:p>\n               <jats:p>5. Except at low lactate concentrations, non-ionic diffusion probably does not play a major role in the entry of lactate into the hepatocyte.<\/jats:p>","DOI":"10.1042\/cs0550183","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:34:59Z","timestamp":1438943699000},"page":"183-188","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mechanism of the Effect of Varying Pco2 on Gluconeogenesis from Lactate in the Perfused Rat Liver"],"prefix":"10.1042","volume":"55","author":[{"given":"R. A.","family":"Iles","sequence":"first","affiliation":[{"name":"The Metabolic and Endocrine Unit, The London Hospital Medical College, University of London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. G.","family":"Baron","sequence":"additional","affiliation":[{"name":"The Metabolic and Endocrine Unit, The London Hospital Medical College, University of London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. D.","family":"Cohen","sequence":"additional","affiliation":[{"name":"The Metabolic and Endocrine Unit, The London Hospital Medical College, University of London"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/55\/2\/183\/450611\/cs0550183.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/55\/2\/183\/450611\/cs0550183.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T01:39:12Z","timestamp":1571967552000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/55\/2\/183\/71084\/Mechanism-of-the-Effect-of-Varying-Pco2-on"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1978,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0550183","relation":{},"ISSN":["0143-5221"],"issn-type":[{"value":"0143-5221","type":"print"}],"subject":[],"published":{"date-parts":[[1978,8,1]]}}}