{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T16:55:51Z","timestamp":1648832151245},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1978,9,1]]},"abstract":"<jats:p>1. Portions of jejunal biopsy specimens from patients with coeliac disease were homogenized in isotonic sucrose solution and subjected to analytical subcellular fractionation by sucrose-density-gradient centrifugation.<\/jats:p>\n               <jats:p>2. The activities and distribution of marker enzymes for the principal subcellular organelles were determined by highly sensitive enzymic micro-assays employing fluorigenic and radiolabelled substrates.<\/jats:p>\n               <jats:p>3. In untreated coeliac disease the brush border showed decreased activities of five marker enzymes, which were reduced to up to one-eighth of control values. On sucrose density gradients the distinct brush-border peak at density 1.21 was replaced by a broad peak at density 1.16.<\/jats:p>\n               <jats:p>4. Two out of three lysosomal enzymes showed increased activities and there was evidence of enhanced fragility of both lysosomes and peroxisomes.<\/jats:p>\n               <jats:p>5. There were reduced activities of both mitochondrial and cytosol malate dehydrogenase, with increased activities of cytosol lactate dehydrogenase.<\/jats:p>\n               <jats:p>6. The endoplasmic reticulum and basal-lateral membranes were relatively unaffected except that the equilibrium densities were slightly increased and decreased respectively.<\/jats:p>\n               <jats:p>7. The patients were treated by gluten withdrawal and subsequent biopsies examined as above. Biopsies from patients treated for 2\u20134 weeks and who had not yet achieved a morphological improvement showed a partial return to normal of the pathological organelles.<\/jats:p>\n               <jats:p>8. Treated patients with partial villus atrophy showed progressively improved enzyme activities and a partial reappearance of the brush-border peak on the sucrose density gradients.<\/jats:p>\n               <jats:p>9. Patients whose intestinal mucosa had returned to morphological normality after prolonged gluten withdrawal showed a persistent defect in the brush-border enzyme activities. This was particularly striking for \u03b2-glucosidase, which was persistently deficient from the brush-border although approximately one-third of the total activity of this enzyme is normally found in this organelle.<\/jats:p>\n               <jats:p>10. The properties and enzyme activities of lysosomes, peroxisomes, endoplasmic reticulum, mitochondria and cytosol were essentially normal in the biopsies from fully treated patients.<\/jats:p>","DOI":"10.1042\/cs0550285","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:34:59Z","timestamp":1438943699000},"page":"285-292","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Analytical Subcellular Fractionation of Jejunal Biopsy Specimens: Enzyme Activities, Organelle Pathology and Response to Gluten Withdrawal in Patients with Coeliac Disease"],"prefix":"10.1042","volume":"55","author":[{"given":"T. J.","family":"Peters","sequence":"first","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. E.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Wells","sequence":"additional","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/55\/3\/285\/451260\/cs0550285.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/55\/3\/285\/451260\/cs0550285.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,26]],"date-time":"2019-10-26T01:58:05Z","timestamp":1572055085000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/55\/3\/285\/73915\/Analytical-Subcellular-Fractionation-of-Jejunal"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1978,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0550285","relation":{},"ISSN":["0143-5221"],"issn-type":[{"value":"0143-5221","type":"print"}],"subject":[],"published":{"date-parts":[[1978,9,1]]}}}