{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T08:29:51Z","timestamp":1652171391821},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"6","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1979,12,1]]},"abstract":"<jats:p>1. The hepatic organelle pathology of 12 patients with Gilbert's syndrome was studied by analytical subcellular fractionation in combination with enzymic microanalysis of liver biopsy material.<\/jats:p>\n               <jats:p>2. All patients showed increased activities of the endoplasmic reticulum marker enzyme, neutral \u03b1-glucosidase. Seven patients showed a decrease in the modal density of the endoplasmic reticulum, from 1\u00b720 to 1\u00b715 g\/ml. These patients also showed striking hypertrophy of the hepatocyte smooth endoplasmic reticulum on electron microscopy. The remaining five patients showed normal endoplasmic reticulum density distribution with a peak at 1\u00b720 g\/ml, and had normal appearance of the endoplasmic reticulum on electron microscopy.<\/jats:p>\n               <jats:p>3. All patients showed increased activity of three lysosomal marker enzymes: N-acetyl-\u03b2-glucosaminidase, acid phosphatase and \u03b2-glucuronidase. The distribution of these enzymes in the sucrose gradients showed less enzyme in the high-density region of the gradients, indicating a reduced equilibrium density of the lysosomes. Assays of latent and sedimentable N-acetyl-\u03b2-glucosaminidase, a measure of lysosomal integrity, were normal.<\/jats:p>\n               <jats:p>4. Marker enzyme activities and density gradient distribution of other organelles, including plasma membrane (5\u2032-nucleotidase), mitochondria (malate dehydrogenase), biliary canaliculi (\u03b3-glutamyl transferase) and cytosol (lactate dehydrogenase) were normal. Increased catalase activities were noted.<\/jats:p>","DOI":"10.1042\/cs0570491","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:36:07Z","timestamp":1438943767000},"page":"491-497","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Gilbert's Syndrome: Analytical Subcellular Fractionation of Liver Biopsy Specimens. Enzyme Activities, Organelle Pathology and Evidence for Subpopulations of the Syndrome"],"prefix":"10.1042","volume":"57","author":[{"given":"J.","family":"Dawson","sequence":"first","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. A.","family":"Seymour","sequence":"additional","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. J.","family":"Peters","sequence":"additional","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/57\/6\/491\/451980\/cs0570491.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/57\/6\/491\/451980\/cs0570491.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T20:02:33Z","timestamp":1638475353000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/57\/6\/491\/72299\/Gilbert-s-Syndrome-Analytical-Subcellular"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1979,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0570491","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,12,1]]}}}