{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T11:22:34Z","timestamp":1789903354002,"version":"4.0.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1980,10,1]]},"abstract":"<jats:p>1. A radioimmunoassay for 25,26-dihydroxycholecalciferol has been developed with an anti-serum raised in a sheep, tritiated 1,25-dihydroxycholecalciferol as tracer and synthetic 25,26-dihydroxycholecalciferol as standard. The metabolite was purified from serum extracts by Sephadex LH 20 and high-pressure liquid chromatography; recovery was monitored with biologically generated, tritiated 25,26-dihydroxycholecalciferol.<\/jats:p>\n               <jats:p>2. The mean \u00b1 sem concentration of 25,26-dihydroxycholecalciferol in serum from 18 healthy subjects was 587 \u00b1 65 pmol\/l. Seven Asian patients with osteomalacia due to vitamin D deficiency had very low or undetectable (&amp;lt; 96\u2013231 pmol\/l) circulating 25,26-dihydroxycholecalciferol concentrations.<\/jats:p>\n               <jats:p>3. The metabolite was detectable in the sera from seven anephric patients (mean 262 \u00b1 43 pmol\/l), indicating that extrarenal sites for the 26-hydroxylation of 25-hydroxycholecalciferol exist in man.<\/jats:p>\n               <jats:p>4. A strong positive correlation between the concentrations of 25-hydroxycholecalciferol and those of 25,26-dihydroxycholecalciferol in serum was obtained. Thus it appears that in man the production of this dihydroxy metabolite of vitamin D depends on the concentration of its precursor, 25-hydroxycholecalciferol.<\/jats:p>","DOI":"10.1042\/cs0590257","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:36:24Z","timestamp":1438943784000},"page":"257-263","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Determination of Circulating 25,26-Dihydroxycholecalciferol in Man by Radioimmunoassay"],"prefix":"10.1042","volume":"59","author":[{"given":"L. J.","family":"Fraher","sequence":"first","affiliation":[{"name":"Department of Medicine, The Middlesex Hospital, London"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. L.","family":"Clemens","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Middlesex Hospital, London"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. E.","family":"Papapoulos","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Middlesex Hospital, London"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Redel","sequence":"additional","affiliation":[{"name":"Institute de Rhumatologie, H\u00f4pital Cochin, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L. H.","family":"O'Riordan","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Middlesex Hospital, London"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/59\/4\/257\/450773\/cs0590257.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/59\/4\/257\/450773\/cs0590257.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T20:08:39Z","timestamp":1638475719000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/59\/4\/257\/72498\/Determination-of-Circulating-25-26"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1980,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0590257","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,10,1]]}}}