{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T22:20:51Z","timestamp":1757542851038},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"6","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1983,12,1]]},"abstract":"<jats:p>1. Low ATP\/ADP ratios have been reported consistently for nucleotide levels of mononuclear cells separated from peripheral blood by conventional techniques.<\/jats:p>\n               <jats:p>2. We have established that these low values (mean 2.3:1) were not due to cell damage or poor viability, but resulted from heavy platelet contamination, which is unavoidable when heparinized blood is used. The results reflect the low ATP\/ADP ratios (mean 1.6:1) characteristic of platelets. Platelet-free extracts from defibrinated blood had very high ATP\/ADP ratios (mean 17.4:1).<\/jats:p>\n               <jats:p>3. The initial finding of detectable amounts of deoxy-ATP and deoxy-GTP in mononuclear cells from children with two distinct inherited immunodeficiency disorders [adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) deficiency respectively] many have been due to contamination by nucleated erythrocytes as well as platelets in non-defibrinated preparations.<\/jats:p>\n               <jats:p>4. Defibrination before nucleotide extraction of mononuclear cells from a patient with T-cell leukaemic\/lymphoma treated with the ADA inhibitor deoxycoformycin enabled the demonstration of grossly raised deoxy-ATP levels relative to deoxy-ADP levels (ratio 16.1:1), associated with severe ATP depletion. This reciprocal relationship between ATP and dATP was found by us previously in the erythrocytes in inherited ADA deficiency.<\/jats:p>\n               <jats:p>5. These findings underline the importance of extracts uncontaminated by platelets, or nucleated erythrocytes, in the evaluation of lymphocyte nucleotide levels in inherited or acquired immunodeficiency syndromes.<\/jats:p>","DOI":"10.1042\/cs0650635","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:46:18Z","timestamp":1438944378000},"page":"635-643","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Importance of Platelet-Free Preparations for Evaluating Lymphocyte Nucleotide Levels in Inherited or Acquired Immunodeficiency Syndromes"],"prefix":"10.1042","volume":"65","author":[{"given":"A.","family":"Goday","sequence":"first","affiliation":[{"name":"Purine Laboratory, Guy's Hospital Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. A.","family":"Simmonds","sequence":"additional","affiliation":[{"name":"Purine Laboratory, Guy's Hospital Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. R.","family":"Webster","sequence":"additional","affiliation":[{"name":"Purine Laboratory, Guy's Hospital Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Levinsky","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute for Child Health, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. R.","family":"Watson","sequence":"additional","affiliation":[{"name":"Department of Paediatric Nephrology, Hospital for Sick Children, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. V.","family":"Hoffbrand","sequence":"additional","affiliation":[{"name":"Department of Haematology, Royal Free Hospital, London"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/65\/6\/635\/454930\/cs0650635.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/65\/6\/635\/454930\/cs0650635.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T20:27:35Z","timestamp":1638476855000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/65\/6\/635\/73235\/Importance-of-Platelet-Free-Preparations-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1983,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0650635","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,12,1]]}}}