{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T20:38:17Z","timestamp":1697920697923},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1985,3,1]]},"abstract":"<jats:p>1. An adenylate cyclase bioassay for parathyroid hormone (PTH) was evaluated in peripheral sera of ten normal subjects, 15 patients with various types of hypoparathyroidism, nine patients with malignancy-associated hypercalcaemia and 60 patients with primary or secondary hyperparathyroidism. The lower limit of detectability was 150 pg\/ml in terms of synthetic human PTH (1-34).<\/jats:p>\n               <jats:p>2. PTH-like bioactivity was not detected in normal subjects, in patients with surgical hypoparathyroidism, in patients with idiopathic hypoparathyroidism or in patients with malignancy-associated hypercalcaemia. Serum from one untreated pseudohypoparathyroid patient consistently contained PTH-like bioactivity.<\/jats:p>\n               <jats:p>3. Bioactivity was detected in sera from 50% of patients with primary or secondary hyperparathyroidism. In two patients with secondary hyperparathyroidism calcium infusions suppressed this activity within 5 min.<\/jats:p>\n               <jats:p>4. Both in primary and in secondary hyperparathyroidism there was a significant positive correlation between levels of PTH-like bioactivity, PTH immunoreactivity and the histological severity of the disease.<\/jats:p>","DOI":"10.1042\/cs0680321","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:57:35Z","timestamp":1438945055000},"page":"321-326","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["An adenylate cyclase bioassay for parathyroid hormone: some clinical experiences"],"prefix":"10.1042","volume":"68","author":[{"given":"M. S.","family":"Seshadri","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Royal North Shore Hospital, St Leonards, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. L.","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Royal North Shore Hospital, St Leonards, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. R.","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Royal North Shore Hospital, St Leonards, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. S.","family":"Mason","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Royal North Shore Hospital, St Leonards, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Posen","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Royal North Shore Hospital, St Leonards, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/68\/3\/321\/455918\/cs0680321.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/68\/3\/321\/455918\/cs0680321.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T20:37:13Z","timestamp":1638477433000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/68\/3\/321\/73557\/An-adenylate-cyclase-bioassay-for-parathyroid"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1985,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0680321","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,3,1]]}}}