{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T02:30:50Z","timestamp":1746325850586},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1985,4,1]]},"abstract":"<jats:p>1. Cyclic AMP accumulation has been measured in whole human sweat glands. The mean rate in glands from 19 subjects was 0.519 \u00b1 0.316 pmol of cyclic AMP formed 5 min\u22121 \u03bcg\u22121 of DNA, which is comparable with that reported for other tissues.<\/jats:p>\n               <jats:p>2. Cyclic AMP accumulation in the sweat gland is stimulated fourfold by prostaglandin (PG) E1 and fivefold by PGE2 (0.1 mmol\/l), in accord with stimulation in renal tubules and medullary membranes.<\/jats:p>\n               <jats:p>3. Bradykinin (10 \u03bcg\/ml) increases the rate threefold and this is substantially prevented by indomethacin (1.5 \u00d7 10\u22125 mol\/l), as also is a five-fold stimulation by cyclic GMP (10\u22125 mol\/l).<\/jats:p>\n               <jats:p>4. Mecholyl (10\u22122 mol\/l) and isoprenaline (6 \u00d7 10\u22126 mol\/l) increase the rate five- and four-fold respectively, and these agonist effects are largely abolished by atropine and propranolol.<\/jats:p>\n               <jats:p>5. The stimulation and inhibition pattern suggests a direct action of PGE, enhancement of prostaglandin synthetase by cyclic GMP and stimulation of guanylate cyclase by mecholyl and bradykinin. Isoprenaline presumably stimulates adenylate cyclase directly.<\/jats:p>\n               <jats:p>6. This complex chain of events, from cholinergic stimulation to an enhancement of adenylate cyclase, demonstrated in vitro, constitutes a potential for flexible and fine control of sweat gland function.<\/jats:p>","DOI":"10.1042\/cs0680433","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:57:35Z","timestamp":1438945055000},"page":"433-439","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The human eccrine sweat gland adenylate cyclase system: response to agonists"],"prefix":"10.1042","volume":"68","author":[{"given":"A. K.","family":"Khullar","sequence":"first","affiliation":[{"name":"Departments of Chemical Pathology and Child Health, University of Manchester, Manchester, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Departments of Chemical Pathology and Child Health, University of Manchester, Manchester, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. D.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Departments of Chemical Pathology and Child Health, University of Manchester, Manchester, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/68\/4\/433\/451200\/cs0680433.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/68\/4\/433\/451200\/cs0680433.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T20:38:17Z","timestamp":1638477497000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/68\/4\/433\/73606\/The-human-eccrine-sweat-gland-adenylate-cyclase"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1985,4,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0680433","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,4,1]]}}}