{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:50:47Z","timestamp":1760215847342},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1987,4,1]]},"abstract":"<jats:p>1. Cytosolic free calcium concentrations ([a2+]i) were measured in resting and chemotactic peptide-activated neutrophils from eight patients with Bartter's syndrome and compared with levels determined in neutrophils isolated from healthy controls.<\/jats:p>\n               <jats:p>2. [Ca2+]i was measured with the intracellular trappable fluorescent indicator Quin2. The synthetic tripeptide formylmethionyl-leucyl-phenylalanine (fMet-Leu-Phe) was used as a stimulant.<\/jats:p>\n               <jats:p>3. No difference was found in resting [Ca2+]i between neutrophils from normal controls and those from patients with Bartter's syndrome.<\/jats:p>\n               <jats:p>4. On the contrary increases in [Ca2+]i stimulated by fMet-Leu-Phe concentrations higher than 10\u22128 mol\/l were significantly less in neutrophils from patients with Bartter's syndrome.<\/jats:p>\n               <jats:p>5. It is suggested that neutrophils from patients affected by Bartter's syndrome exhibit an intrinsic anomaly in the mechanism responsible for intracellular Ca2+ mobilization.<\/jats:p>","DOI":"10.1042\/cs0720483","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:11:45Z","timestamp":1438945905000},"page":"483-488","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Resting and stimulated cytosolic free calcium levels in neutrophils from patients with Bartter's syndrome"],"prefix":"10.1042","volume":"72","author":[{"given":"Francesco","family":"Di Virgilio","sequence":"first","affiliation":[{"name":"C.N.R. Unit for the Study of the Physiology of Mitochondria and Institute of General Pathology, Padova"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenzo","family":"Cal\u00f2","sequence":"additional","affiliation":[{"name":"Institute of Internal Medicine, Postgraduate School of Nephrology, University Hospital, Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvatore","family":"Cantaro","sequence":"additional","affiliation":[{"name":"Institute of Internal Medicine, Postgraduate School of Nephrology, University Hospital, Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Favaro","sequence":"additional","affiliation":[{"name":"Institute of Internal Medicine, Postgraduate School of Nephrology, University Hospital, Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Piccoli","sequence":"additional","affiliation":[{"name":"Institute of Internal Medicine, Postgraduate School of Nephrology, University Hospital, Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arturo","family":"Borsatti","sequence":"additional","affiliation":[{"name":"Institute of Internal Medicine, Postgraduate School of Nephrology, University Hospital, Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/72\/4\/483\/457666\/cs0720483.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/72\/4\/483\/457666\/cs0720483.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T20:45:03Z","timestamp":1638477903000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/72\/4\/483\/74173\/Resting-and-stimulated-cytosolic-free-calcium"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,4,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0720483","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,4,1]]}}}