{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:56:21Z","timestamp":1773662181124,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,3,1]]},"abstract":"<jats:p>1. For the diagnosis of electrolyte disorders, data on skeletal muscle composition are often valuable, but rarely available. We have therefore developed a simple and rapid needle biopsy procedure for the determination of the concentrations of K+, Na+, Mg2+ and Na+, K+-pumps in muscle.<\/jats:p>\n               <jats:p>2. Using a Bergstr\u00f6m needle, biopsies weighing around 25 mg were taken from the vastus lateralis muscle of 18 normal subjects (aged 44\u201386 years) and extracted with trichloroacetic acid (TCA). The concentrations of K+, Na+ and Mg2+ were 90.7 \u00b1 1.8, 31.9 \u00b1 1.6 and 9.5 \u00b1 0.2 \u03bcmol\/g wet wt., respectively (means \u00b1 se).<\/jats:p>\n               <jats:p>3. The TCA extraction gave the same values as digestion with 65% HNO3 or 35% H2O2, could be used over the weight range 10\u201355 mg and showed a Mg2+ recovery of 101.7%.<\/jats:p>\n               <jats:p>4. The concentration of Na+, K+-pumps was quantified as the total capacity for [3H]ouabain binding. In vastus lateralis biopsies obtained from six normal subjects the mean value was 258 \u00b1 16 pmol\/g wet wt.<\/jats:p>\n               <jats:p>5. Comparison of the concentrations of K+, Mg2+ and [3H]ouabain-binding sites in samples obtained post mortem showed modest variation among different muscles with varying fibre composition.<\/jats:p>\n               <jats:p>6. Measurements of the concentrations of K+, Na+, Mg2+ and Na+, K+-pumps in duplicate biopsies of the vastus lateralis yield values which seem representative for the total pool of skeletal muscle fibres and can be performed within a few hours.<\/jats:p>\n               <jats:p>7. Measurements on patients receiving chronic diuretic treatment demonstrated that these procedures allow the detection of reduced levels of K+, Mg2+ and Na+, K+-pumps in skeletal muscle, as well as a partial recovery after oral Mg2+ supplementation.<\/jats:p>","DOI":"10.1042\/cs0740241","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:18:52Z","timestamp":1438946332000},"page":"241-248","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["A simple and rapid method for the determination of the concentrations of magnesium, sodium, potassium and sodium, potassium pumps in human skeletal muscle"],"prefix":"10.1042","volume":"74","author":[{"given":"Inge","family":"D\u00f8rup","sequence":"first","affiliation":[{"name":"1Department of Internal Medicine, Odder Sygehus Odder, \u00c5rhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristjar","family":"Skajaa","sequence":"additional","affiliation":[{"name":"3Department of Internal Medicine, Odder Sygehus Odder, \u00c5rhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torben","family":"Clausen","sequence":"additional","affiliation":[{"name":"Institute of Physiology, Aarhus University, \u00c5rhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/74\/3\/241\/458890\/cs0740241.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/74\/3\/241\/458890\/cs0740241.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:23:15Z","timestamp":1638397395000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/74\/3\/241\/74305\/A-simple-and-rapid-method-for-the-determination-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0740241","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,3,1]]}}}