{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T17:25:30Z","timestamp":1648833930800},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"6","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,6,1]]},"abstract":"<jats:p>1. Endogenous noradrenaline release from washed platelets incubated under resting conditions and in the presence of thrombin was examined in 14 normal subjects and 10 subjects with type 1 (insulin-dependent) diabetes.<\/jats:p>\n               <jats:p>2. Irreversible aggregation of platelets from both normal and diabetic subjects was induced by thrombin (0.3 unit\/ml). Platelets from diabetic subjects were more sensitive than platelets from normal subjects, extents of aggregation being 89% and 76%, respectively (P &amp;lt; 0.002).<\/jats:p>\n               <jats:p>3. Stimulation with thrombin (0.3 unit\/ml) elicited marked platelet release of noradrenaline to the incubation medium in both normal and diabetic subjects. Supernatant noradrenaline concentrations obtained under thrombin-stimulated conditions did not significantly differ between normal and diabetic subjects. However, under resting conditions noradrenaline levels were significantly greater (+ 93%, P &amp;lt; 0.02) for diabetic than normal subjects.<\/jats:p>\n               <jats:p>4. Measurement of platelet noradrenaline contents after thrombin stimulation revealed no difference between normal and diabetic subjects. Under resting conditions, however, platelet noradrenaline levels were significantly lower (\u221246%, P &amp;lt; 0.02) for diabetic than normal subjects. Thus, in the diabetic subjects increased resting platelet efflux of noradrenaline is mirrored by a decreased platelet noradrenaline content.<\/jats:p>\n               <jats:p>5. A consequence of increases in resting catecholamine efflux may be enhanced platelet activity resulting in increased platelet aggregation.<\/jats:p>","DOI":"10.1042\/cs0760603","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:27:12Z","timestamp":1438946832000},"page":"603-607","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Platelet efflux of noradrenaline in patients with type 1 diabetes mellitus"],"prefix":"10.1042","volume":"76","author":[{"given":"C. C. T.","family":"Smith","sequence":"first","affiliation":[{"name":"Cardiovascular Research Laboratory, Departments of Medicine and Clinical Pharmacology, University College and Middlesex School of Medicine, University College London, The Rayne Institute, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. P.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Laboratory, Departments of Medicine and Clinical Pharmacology, University College and Middlesex School of Medicine, University College London, The Rayne Institute, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. N. C.","family":"Prichard","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Laboratory, Departments of Medicine and Clinical Pharmacology, University College and Middlesex School of Medicine, University College London, The Rayne Institute, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. J.","family":"Betteridge","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Laboratory, Departments of Medicine and Clinical Pharmacology, University College and Middlesex School of Medicine, University College London, The Rayne Institute, London"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/76\/6\/603\/508156\/cs0760603.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/76\/6\/603\/508156\/cs0760603.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:27:18Z","timestamp":1638397638000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/76\/6\/603\/74523\/Platelet-efflux-of-noradrenaline-in-patients-with"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1989,6,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0760603","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,6,1]]}}}