{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T18:50:51Z","timestamp":1773427851484,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,10,1]]},"abstract":"<jats:p>1. Sera from 76 patients with primary biliary cirrhosis (PBC) and 66 control subjects (53 with chronic liver disease and 13 healthy normal women) were immunoblotted against purified E1 component of bovine pyruvate dehydrogenase complex (PDC) and bacterial PDC.<\/jats:p>\n               <jats:p>2. Thirty-one out of seventy-six (41%) sera from PBC patients showed a positive response to bovine E1\u03b1, and five of these 31 (7% of total) reacted with bovine E1\u03b2. None of the control sera reacted with bovine E1 \u03b1 or \u03b2.<\/jats:p>\n               <jats:p>3. None of the PBC sera that recognized bovine E1 subunits reacted with bacterial PDC E1.<\/jats:p>\n               <jats:p>4. In the PBC patients there was no correlation between presence of antibodies to E1\u03b1 and \u03b2 subunits and histological stage of the disease.<\/jats:p>\n               <jats:p>5. Our data demonstrate that the E1\u03b1 and \u03b2 components of mammalian PDC are the M2\u2018d\u2019 and \u2018e\u2019 mitochondrial autoantigens, respectively.<\/jats:p>","DOI":"10.1042\/cs0770365","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:29:18Z","timestamp":1438946958000},"page":"365-368","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["The E1 \u03b1 and <i>\u03b2<\/i> Subunits of the Pyruvate Dehydrogenase Complex are M2\u2018d\u2019 and M2\u2018e\u2019 Autoantigens in Primary Biliary Cirrhosis"],"prefix":"10.1042","volume":"77","author":[{"given":"Shelley P. M.","family":"Fussey","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Genetics, Newcastle upon Tyne, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret F.","family":"Bassendine","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothy","family":"Fittes","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Genetics, Newcastle upon Tyne, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian B.","family":"Turner","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver F. W.","family":"James","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen J.","family":"Yeaman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Genetics, Newcastle upon Tyne, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/77\/4\/365\/459951\/cs0770365.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/77\/4\/365\/459951\/cs0770365.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:29:31Z","timestamp":1638397771000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/77\/4\/365\/74823\/The-E1-and-Subunits-of-the-Pyruvate-Dehydrogenase"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0770365","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,10,1]]}}}