{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T08:56:22Z","timestamp":1759481782604},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,11,1]]},"abstract":"<jats:p>1. Plasma levels of immunoreactive N-terminal pro-atrial natriuretic peptide (N-terminal ANP) have been measured in 25 normal subjects, 29 patients with essential hypertension, six cardiac transplant recipients, seven patients with dialysis-independent chronic renal failure and 11 patients with haemodialysis-dependent chronic renal failure. Plasma was extracted on Sep-Pak cartridges and N-terminal ANP immunoreactivity was measured using an antibody directed against pro-ANP (1\u201330).<\/jats:p>\n               <jats:p>2. Plasma levels of TV-terminal ANP (means \u00b1 sem) were 235.3 \u00b1 19.2 pg\/ml in normal subjects and were significantly raised in patients with essential hypertension (363.6 \u00b1 36.3 pg\/ml), in cardiac transplant recipients (1240.0 \u00b1 196.2 pg\/ml), in patients with chronic renal failure not requiring dialysis (1636.6 \u00b1 488.4 pg\/ml) and patients with chronic renal failure on maintenance haemodialysis (10 336.1 \u00b1 2043.7 pg\/ml).<\/jats:p>\n               <jats:p>3. There were positive and significant correlations between the plasma levels of TV-terminal ANP and \u03b1-human ANP (\u03b1-hANP) with individual correlation coefficients of 0.68 within the normal subjects, 0.47 in patients with essential hypertension, 0.78 in patients with dialysis-independent chronic renal failure and 0.68 in patients with haemodialysis-dependent chronic renal failure (P &amp;lt; 0.05 in every case).<\/jats:p>\n               <jats:p>4. Gel filtration behaviour on Sephadex G-50 of the immunoreactive N-terminal ANP from Sep-Pak extracts of plasma from normal subjects or patients was consistent with a single peak having an elution volume corresponding to that of human pro-ANP (1\u201367) standard.<\/jats:p>\n               <jats:p>5. These studies demonstrate that the N-terminal pro-ANP peptide is co-secreted with \u03b1-hANP in both normal subjects and patients with cardiovascular\/renal disease. The higher levels of the N-terminal ANP may reflect differences in the rate of elimination from the circulation but the exact structure and functional significance of the circulating N-terminal ANP remains to be established.<\/jats:p>","DOI":"10.1042\/cs0770573","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:29:18Z","timestamp":1438946958000},"page":"573-579","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Immunoreactive <i>N<\/i>-terminal Pro-Atrial Natriuretic Peptide in Human Plasma: Plasma Levels and Comparisons with \u03b1-Human Atrial Natriuretic Peptide in Normal Subjects, Patients with Essential Hypertension, Cardiac Transplant and Chronic Renal Failure"],"prefix":"10.1042","volume":"77","author":[{"given":"M. G.","family":"Buckley","sequence":"first","affiliation":[{"name":"Blood Pressure Unit, Department of Medicine, Charing Cross and Westminster Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. A.","family":"Sagnella","sequence":"additional","affiliation":[{"name":"Blood Pressure Unit, Department of Medicine, Charing Cross and Westminster Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. D.","family":"Markandu","sequence":"additional","affiliation":[{"name":"Blood Pressure Unit, Department of Medicine, Charing Cross and Westminster Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. R. J.","family":"Singer","sequence":"additional","affiliation":[{"name":"Blood Pressure Unit, Department of Medicine, Charing Cross and Westminster Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. A.","family":"MacGregor","sequence":"additional","affiliation":[{"name":"Blood Pressure Unit, Department of Medicine, Charing Cross and Westminster Medical School, London"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/77\/5\/573\/460694\/cs0770573.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/77\/5\/573\/460694\/cs0770573.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:31:09Z","timestamp":1638397869000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/77\/5\/573\/74952\/Immunoreactive-N-terminal-Pro-Atrial-Natriuretic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1989,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0770573","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,11,1]]}}}