{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T17:49:20Z","timestamp":1693504160022},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,10,1]]},"abstract":"<jats:p>1. Calcitriol (1,25-dihydroxyvitamin D3) concentrations in plasma of humans and pigs with pseudo-vitamin D deficiency rickets type I (PVDRI) have been reported to be significantly lower than in normal subjects and animals. Sometimes, however, calcitriol concentrations are relatively high in these subjects and animals (50\u201380 pmol\/l) and nevertheless clinical symptoms of rickets develop. We have studied whether or not the development of rachitic lesions in piglets with PVDRI is due to altered binding properties of the intestinal calcitriol receptor in addition to the defective renal production of calcitriol. PVDRI piglets with clinical and biochemical symptoms of rickets (hypocalcaemia, increased activity of alkaline phosphatase) and with calcitriol concentrations in plasma of 83.7 \u00b1 4.2 pmol\/l (n = 7) were used. They were compared with unaffected piglets with normal calcitriol concentrations (178.0 \u00b1 17.7 pmol\/l, n = 9).<\/jats:p>\n               <jats:p>2. The equilibrium dissociation constant (Kd) of the receptor in the PVDRI piglets (0.31 \u00b1 0.05 nmol\/l) and in control piglets (0.33 \u00b1 0.05 nmol\/l) and the maximum binding capacity (Bmax.) (674 \u00b1 103 and 719 \u00b1 122 fmol\/mg of protein, respectively) were not different (n = 9).<\/jats:p>\n               <jats:p>3. The association rate constant (kass) at 4\u00b0C [0.15 \u00d7 107 and 0.24 \u00d7 107 (mol\/l)\u22121 min\u22121] and the dissociation rate constant (kdiss) (0.40 \u00d7 10\u22123 and 0.48 \u00d7 10\u22123 min\u22121; half-life of dissociation = 24.1 and 28.9 h, respectively) were also not different between diseased and control piglets.<\/jats:p>\n               <jats:p>4. No differences between PVDRI and control piglets were also found for the relative molecular mass (47 500 and 47700, respectively) and the Stokes' radius (3.04 and 3.05 nm, respectively) of the calcitriol receptor.<\/jats:p>\n               <jats:p>5. It is concluded that the intestinal calcitriol receptor of this animal model functions normally and that changes in binding properties and concentration of the intestinal calcitriol receptor do not contribute to the development of rachitic lesions in PVDRI piglets.<\/jats:p>","DOI":"10.1042\/cs0790409","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:30:05Z","timestamp":1438947005000},"page":"409-414","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Studies of the Porcine Intestinal Calcitriol Receptor in Pseudo-Vitamin D Deficiency Rickets Type I"],"prefix":"10.1042","volume":"79","author":[{"given":"B.","family":"Schr\u00f6der","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Veterinary Medicine, Hannover, F.R.G."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Kaune","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Veterinary Medicine, Hannover, F.R.G."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Harmeyer","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Veterinary Medicine, Hannover, F.R.G."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/79\/4\/409\/462343\/cs0790409.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/79\/4\/409\/462343\/cs0790409.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:32:43Z","timestamp":1638397963000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/79\/4\/409\/75065\/Studies-of-the-Porcine-Intestinal-Calcitriol"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1990,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0790409","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,10,1]]}}}