{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T17:46:45Z","timestamp":1758476805048},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,9,1]]},"abstract":"<jats:p>1. Research into the pathogenesis of acute and chronic neonatal lung disease has been hampered by the lack of a suitable small-animal model of prematurity. We describe such a model that has been developed and validated in the guinea-pig.<\/jats:p>\n               <jats:p>2. Pre-term guinea-pigs delivered by Caesarian section at 65 days gestation (normal gestation 68 days) exhibited transient respiratory distress. The survival of pre-term animals was lower than that of term animals after exposure to 95% O2 (pre-term 42% versus term 79% at 96 h, P &amp;lt; 0.05).<\/jats:p>\n               <jats:p>3. Pulmonary histology in pre-term animals exposed to both 21% O2 and 95% O2 revealed evidence of acute lung injury with atelectasis, pulmonary oedema, fibrin deposition and inflammatory cell infiltration. No evidence of lung injury was observed in term animals exposed to 21% O2, whereas those exposed to 95% O2 showed a similar, but less pronounced, injury to that seen in preterm pups.<\/jats:p>\n               <jats:p>4. The protein concentration in bronchoalveolar lavage fluid was similar in pre-term and term animals exposed to 95% O2, but neutrophil numbers in bronchoalveolar lavage fluid tended to be greater in preterm pups.<\/jats:p>\n               <jats:p>5. Elastase-like activity, measured against succinyl-1-trialanine p-nitroanilide, was higher in bronchoalveolar lavage fluid from control pre-term animals compared with that from control term animals. Exposure to 95% O2 increased the elastase-like activity significantly in both groups. The majority of the elastase-like activity was EDTA-sensitive and thus is possibly due to metallo-elastase. Fractionation of bronchoalveolar lavage fluid indicated that the elastase-like activity was associated with a high-molecular-mass complex. Lipase treatment reduced the activity of this fraction and generated a new 40 kDa fraction.<\/jats:p>\n               <jats:p>6. We conclude that the pre-term guinea-pig is more susceptible than the term animal to lung injury after O2 exposure and thus represents a appropriate small-animal model in which to investigate the pathogenesis of acute and chronic lung injury in the pre-term infant.<\/jats:p>","DOI":"10.1042\/cs0810439","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:33:17Z","timestamp":1438947197000},"page":"439-446","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["The pre-term guinea-pig: a model for the study of neonatal lung disease"],"prefix":"10.1042","volume":"81","author":[{"given":"F. J.","family":"Kelly","sequence":"first","affiliation":[{"name":"Department of Human Nutrition, University of Southampton, Southampton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. I.","family":"Town","sequence":"additional","affiliation":[{"name":"Department of Immunopharmacology, University of Southampton, Southampton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. J.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, University of Southampton, Southampton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. T.","family":"Holgate","sequence":"additional","affiliation":[{"name":"Department of Immunopharmacology, University of Southampton, Southampton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. R.","family":"Roche","sequence":"additional","affiliation":[{"name":"\u2020 Department of Pathology, University of Southampton, Southampton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. D.","family":"Postle","sequence":"additional","affiliation":[{"name":"Department of Child Health, University of Southampton, Southampton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/81\/3\/439\/463156\/cs0810439.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/81\/3\/439\/463156\/cs0810439.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:38:46Z","timestamp":1638398326000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/81\/3\/439\/75604\/The-pre-term-guinea-pig-a-model-for-the-study-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0810439","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,9,1]]}}}