{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:19:35Z","timestamp":1761059975987},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,10,1]]},"abstract":"<jats:p>1. The effects of spermine in the concentration range 0\u201310 mmol\/l on (a) the fluid absorption, (b) the polyethylene glycol permeability, (c) the release of collagenase activity activity into the lumen and (d) the histological appearance of rat descending colon were examined.<\/jats:p>\n               <jats:p>2. Spermine (5 mmol\/l) decreased fluid absorption from 48.83 \u00b1 2.98 (n = 7) to 23.98 \u00b1 2.32 (n = 6) \u03bcl h\u22121 cm\u22122 (P&amp;lt;0.01); polyethylene glycol 4000 permeability was increased from 0.030 \u00b1 0.001 (n = 7) to 0.047 \u00b1 0.003 (n = 6) cm\/h (P&amp;lt;0.01) and luminal collagenase activity increased from a negligible control value to 250 \u00b1 39 (n = 6) units\/ml (P&amp;lt;0.001). Spermine also caused oedema formation within the mucosal interstitial fluid, without inducing an overt breakdown of the mucosa at the luminal surface.<\/jats:p>\n               <jats:p>3. Polyamine-free dialysed seminal plasma had no effect on polyethylene glycol 4000 permeability, although it still caused a significant decrease in colonic fluid absorption from 48.83 \u00b1 2.98 (n = 7) (control) to 31.41 \u00b1 2.08 (n = 5) \u03bcl h\u22121 cm\u22122 (P&amp;lt;0.01).<\/jats:p>\n               <jats:p>4. Low-molecular-mass heparin (600 units\/ml) prevented the spermine (5 mmol\/l)- and whole-semen-induced increase in colonic polyethylene glycol 4000 permeability and reduced the effect of semen on fluid absorption by 63% (P&amp;lt;0.001) and that of spermine by 56% (P&amp;lt;0.01).<\/jats:p>\n               <jats:p>5. The Zn2+ chelator and collagenase inhibitor o-phenanthroline reduced the effect of spermine on fluid absorption and polyethylene glycol 4000 permeability by 100% (P&amp;lt;0.001) and on interstitial oedema formation. o-Phenanthroline also reduced the effects of whole semen on fluid absorption (by 70%, P&amp;lt;0.01) and on polyethylene glycol 4000 permeability by 95%, P&amp;lt;0.01).<\/jats:p>\n               <jats:p>6. A synthetic peptide inhibitor of mammalian collagenase activity with high affinity reduced the effects of whole semen on polyethylene glycol 4000 permeability (P&amp;lt;0.01) and on fluid absorption (P&amp;lt;0.01) in a dose-dependent manner. The inhibitor (2 mmol\/l) also reduced the effects of spermine (5 mmol\/l) on fluid absorption and polyethylene glycol 4000 permeability.<\/jats:p>\n               <jats:p>7. These results indicate that polyamines that are present within semen in the concentration range 5\u201315 mmol\/l act co-operatively with seminal collagenases to disrupt the colonic barrier. Cationic polyamines may activate collagenases by binding to and neutralizing the anionic charge of heparins within the interstitial matrix.<\/jats:p>","DOI":"10.1042\/cs0830417","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:34:06Z","timestamp":1438947246000},"page":"417-423","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effects of spermine on water absorption, polyethylene glycol 4000 permeability and collagenase activity in rat descending colon <i>in vivo<\/i>"],"prefix":"10.1042","volume":"83","author":[{"given":"M. V.","family":"Mendizabal","sequence":"first","affiliation":[{"name":"Division of Biomedical Sciences, Physiology Group, King's College London, London, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Naftalin","sequence":"additional","affiliation":[{"name":"Division of Biomedical Sciences, Physiology Group, King's College London, London, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/83\/4\/417\/463734\/cs0830417.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/83\/4\/417\/463734\/cs0830417.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:39:53Z","timestamp":1638398393000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/83\/4\/417\/75746\/Effects-of-spermine-on-water-absorption"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0830417","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,10,1]]}}}