{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:08:46Z","timestamp":1757779726966},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,3,1]]},"abstract":"<jats:p>1. We determined glomerular and medullary tumour necrosis factor-\u03b1 mRNA levels in acute puromycin aminonucleoside nephrosis on days 0, 8 and 20.<\/jats:p>\n               <jats:p>2. Tumour necrosis factor-\u03b1 mRNA levels were increased fourfold in glomeruli and twofold in the medulla during the nephrotic stage of acute puromycin aminonucleoside nephrosis (day 8).<\/jats:p>\n               <jats:p>3. The high tumour necrosis factor-\u03b1 mRNA levels in both glomeruli and the medulla were ameliorated significantly by methylprednisolone administration.<\/jats:p>\n               <jats:p>4. Focal glomerular sclerosis was induced in rats by injection of puromycin aminonucleoside on days 0, 27, 34 and 41 and by unilateral nephrectomy on day 22.<\/jats:p>\n               <jats:p>5. The percentage of sclerosing glomeruli was 16.6% on day 48 and had increased significantly to 72.8% on day 80.<\/jats:p>\n               <jats:p>6. During the sclerotic phase of puromycin amino-nucleoside nephrosis, glomerular tumour necrosis factor-\u03b1 mRNA levels increased as glomerular sclerosis progressed. On day 80, glomerular tumour necrosis factor-\u03b1 mRNA levels were 13-fold higher than levels in control rats.<\/jats:p>\n               <jats:p>7. These data suggest that glomerular tumour necrosis factor-\u03b1 mRNA expression is associated with the development of puromycin aminonucleoside-induced glomerular sclerosis.<\/jats:p>","DOI":"10.1042\/cs0840349","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:34:58Z","timestamp":1438947298000},"page":"349-356","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Altered glomerular steady-state levels of tumour necrosis factor-<i>\u03b1<\/i> mRNA during nephrotic and sclerotic phases of puromycin aminonucleoside nephrosis in rats"],"prefix":"10.1042","volume":"84","author":[{"given":"Tsukasa","family":"Nakamura","sequence":"first","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Ebihara","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsumine","family":"Fukui","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshimasa","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiko","family":"Tomino","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikaru","family":"Koide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/84\/3\/349\/464064\/cs0840349.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/84\/3\/349\/464064\/cs0840349.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:41:28Z","timestamp":1638398488000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/84\/3\/349\/75854\/Altered-glomerular-steady-state-levels-of-tumour"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0840349","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,3,1]]}}}