{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:22:03Z","timestamp":1760217723368},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,8,1]]},"abstract":"<jats:p>1. We report some pitfalls in the measurement of whole blood and plasma glutathione in man.<\/jats:p>\n               <jats:p>2. Using a simple tourniquet to the forearm and a 21-gauge needle, blood samples were collected by brachial vein puncture from healthy subjects. Whole blood and plasma were analysed for total glutathione, including the reduced and oxidized forms, by a spectrophotometric recycling method involving the glutathione reductase\/NADPH couple.<\/jats:p>\n               <jats:p>3. The concentration of oxidized glutathione was determined after treatment of aliquots with either 2-vinylpyridine or N-ethylmaleimide to trap reduced glutathione. Reduced glutathione in the native samples could then be obtained by subtraction.<\/jats:p>\n               <jats:p>4. When the reagents were added to separated plasma, 2-vinylpyridine yielded values for oxidized glutathione that were twice as high as with N-ethylmaleimide. In whole blood studies the discrepancy was even greater, and the problem was not resolved by deproteinization of samples with 5-sulphosalicylic acid. Using N-ethylmaleimide, levels of oxidized glutathione were less than 1% of total glutathione in whole blood.<\/jats:p>\n               <jats:p>5. Despite attempts to minimize haemolysis, lysed erythrocytes contributed on average 25% to the \u2018plasma\u2019 glutathione concentration.<\/jats:p>","DOI":"10.1042\/cs0850213","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:35:07Z","timestamp":1438947307000},"page":"213-218","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Some Pitfalls in the Measurement of Blood Glutathione"],"prefix":"10.1042","volume":"85","author":[{"given":"D.","family":"Schofield","sequence":"first","affiliation":[{"name":"Pancreato-Biliary Service, Royal Infirmary, Manchester, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Mei","sequence":"additional","affiliation":[{"name":"Pancreato-Biliary Service, Royal Infirmary, Manchester, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"Braganza","sequence":"additional","affiliation":[{"name":"Pancreato-Biliary Service, Royal Infirmary, Manchester, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/85\/2\/213\/464312\/cs0850213.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/85\/2\/213\/464312\/cs0850213.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:42:27Z","timestamp":1638398547000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/85\/2\/213\/75930\/Some-Pitfalls-in-the-Measurement-of-Blood"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0850213","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"type":"print","value":"0143-5221"},{"type":"electronic","value":"1470-8736"}],"subject":[],"published":{"date-parts":[[1993,8,1]]}}}