{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:36:46Z","timestamp":1782779806789,"version":"3.54.5"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,4,1]]},"abstract":"<jats:p>1. The concentration of alkali-soluble protein, DNA and RNA in percutaneous muscle biopsy specimens was analysed. Tissue alkali-soluble protein\/DNA ratio is a measure of muscle protein concentration, while tissue RNA\/DNA ratio may reflect the capacity for protein synthesis.<\/jats:p>\n               <jats:p>2. Patients with weight loss due to cancer (n = 6) were compared with metabolically healthy patients before elective surgery (n = 7). Alkali-soluble protein\/DNA and RNA\/DNA ratios in the weight loss group were 248 (14) g\/g and 1.3 (0.1) g\/g respectively as compared with 404 (13) g\/g and 2.1 (0.1) g\/g in otherwise healthy patients. All of the alkali-soluble protein\/DNA ratios and 5\/6 of the RNA\/DNA ratios in the weight loss group were below the 95% confidence interval for the healthy control subjects.<\/jats:p>\n               <jats:p>3. Patients undergoing elective open cholecystectomy (n = 7) were studied preoperatively and on days 3, 10, 20 and 30 post-operatively. The alkali-soluble protein\/DNA ratio remained unchanged on postoperative day 3 but decreased by 8.7% (P &amp;lt; 0.01), 9.6% (P &amp;lt; 0.05) and 20.4% (P &amp;lt; 0.01) on days 10, 20 and 30 respectively in patients eating at will after the operation. No significant post-operative changes in alkali-soluble protein\/DNA ratio were seen in patients given post-operative total parenteral nutrition with (n = 9) or without (n = 7) glycyl-glutamine supplementation for 3 days after surgery.<\/jats:p>\n               <jats:p>4. In conclusion, patients with weight loss due to malignant disease have a low muscle protein concentration. Elective surgery of medium magnitude results in a decrease in muscle protein lasting for more than 30 days. This is in contrast to patients receiving post-operative total parenteral nutrition, in whom no significant reduction in muscle protein was noted following surgery regardless of whether or not the total parenteral nutrition was supplemented with glutamine.<\/jats:p>","DOI":"10.1042\/cs0880479","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:37:12Z","timestamp":1438947432000},"page":"479-484","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Human Skeletal Muscle Protein: Effect of Malnutrition, Elective Surgery and Total Parenteral Nutrition"],"prefix":"10.1042","volume":"88","author":[{"given":"Benny","family":"Petersson","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Intensive Care and the Metabolic Research Laboratory at the Karolinska Institute, St G\u00f6rans Hospital, Stockholm, Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Hultman","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry II, Huddinge University Hospital, Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care and the Metabolic Research Laboratory at the Karolinska Institute, St G\u00f6rans Hospital, Stockholm, Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Wernerman","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care and the Metabolic Research Laboratory at the Karolinska Institute, St G\u00f6rans Hospital, Stockholm, Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/88\/4\/479\/465375\/cs0880479.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/88\/4\/479\/465375\/cs0880479.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:50:53Z","timestamp":1638399053000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/88\/4\/479\/76360\/Human-Skeletal-Muscle-Protein-Effect-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,4,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0880479","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,4,1]]}}}