{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:05:15Z","timestamp":1787238315520,"version":"build-2736575974"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,7,1]]},"abstract":"<jats:p>1. As lipopolysaccharide is a major stimulator of neutrophil responses during Gram-negative bacterial infections, we studied its effect on the membrane expression of neutral endopeptidase 24.11\/CD10 on neutrophils in a model of endotoxaemia in vitro. Lipopolysaccharide added to human whole-blood induced a marked and sustained CD10\/neutral endopeptidase upregulation that was already detectable at 0.1 ng\/ml and was maximal at a lipopolysaccharide concentration of 10 ng\/ml.<\/jats:p>\n                  <jats:p>2. We observed that neither tumour necrosis factor-\u03b1 nor any newly synthesized protein was involved in the upregulation observed after 1 h incubation with 10 ng\/ml lipopolysaccharide.<\/jats:p>\n                  <jats:p>3. We further studied whether the lipopolysaccharide-induced CD10\/neutral endopeptidase upregulation was mediated by lipopolysaccharide binding to the neutrophil CD14 receptor. Incubation of whole blood with an anti-CD14 monoclonal antibody before the addition of 0.1 ng\/ml or 0.5 ng\/ml lipopolysaccharide resulted in complete inhibition of CD10\/neutral endopeptidase upregulation. In contrast, at a lipopolysaccharide concentration of 10 ng\/ml, the anti-CD14 monoclonal antibody had an incomplete blocking effect.<\/jats:p>\n                  <jats:p>4. The differential requirement for the CD14 receptor, depending on the lipopolysaccharide dose, was confirmed by the study of a patient suffering from paroxysmal nocturnal haemoglobinuria (in whom a complete defect in neutrophil CD14 expression was previously documented).<\/jats:p>\n                  <jats:p>5. We finally confirmed these results using purified neutrophils, demonstrating that lipopolysaccharide-induced CD10\/neutral endopeptidase upregulation depends on direct interaction with neutrophil CD14.<\/jats:p>","DOI":"10.1042\/cs0890083","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T07:38:13Z","timestamp":1438933093000},"page":"83-89","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Lipopolysaccharide Induces Upregulation of Neutral Endopeptidase 24.11 on Human Neutrophils: Involvement of the CD14 Receptor"],"prefix":"10.1042","volume":"89","author":[{"given":"Christine","family":"Fagny","sequence":"first","affiliation":[{"name":"Laboratoire Pluridisciplinaire de Recherche Exp\u00e9rimentale Biom\u00e9dicale, Facult\u00e9 de M\u00e9decine, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Marchant","sequence":"additional","affiliation":[{"name":"Service d'Immunologie, H\u00f4pital Erasme, Universit\u00e9 Libre de Bruxelles, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"De Prez","sequence":"additional","affiliation":[{"name":"Laboratoire Pluridisciplinaire de Recherche Exp\u00e9rimentale Biom\u00e9dicale, Facult\u00e9 de M\u00e9decine, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Goldman","sequence":"additional","affiliation":[{"name":"Laboratoire Pluridisciplinaire de Recherche Exp\u00e9rimentale Biom\u00e9dicale, Facult\u00e9 de M\u00e9decine, Brussels, Belgium"},{"name":"Service d'Immunologie, H\u00f4pital Erasme, Universit\u00e9 Libre de Bruxelles, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"Deschodt-Lanckman","sequence":"additional","affiliation":[{"name":"Laboratoire Pluridisciplinaire de Recherche Exp\u00e9rimentale Biom\u00e9dicale, Facult\u00e9 de M\u00e9decine, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/89\/1\/83\/465722\/cs0890083.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/89\/1\/83\/465722\/cs0890083.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T17:21:36Z","timestamp":1638379296000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/89\/1\/83\/73742\/Lipopolysaccharide-Induces-Upregulation-of-Neutral"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0890083","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,7,1]]}}}