{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:06:56Z","timestamp":1758924416989},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"6","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,6,1]]},"abstract":"<jats:p>1. Receptors for angiotensin II were identified and characterized in membrane fractions from myometrial samples obtained at non-pregnant hysterectomy in women of reproductive age. Specific binding was also measured in paired samples of vascular and \u2018vessel-free\u2019 myometrium.<\/jats:p>\n               <jats:p>2. A single class of high-affinity receptor sites was identified in the total myometrial preparations (n = 10), with a median equilibrium dissociation constant (Kd) of 0.122 nmol\/l (range 0.065\u20130.465 nmol\/l) and concentration of receptor sites (Bmax) of 149 fmol\/mg protein (range 105\u2013435 fmol\/mg). Receptor characterization studies using losartan (an angiotensin type 1 receptor antagonist) and PD123177 (an angiotensin type 2 receptor antagonist) showed the myometrium to contain almost entirely type 2 receptors.<\/jats:p>\n               <jats:p>3. The median Kd and Bmax for specific angiotensin II binding in isolated myometrial vessel homogenates were 0.086 nmol\/l (range 0.060\u20130.433) and 260 fmol\/mg protein (range 197\u2013737 fmol\/mg) respectively. Vascular specific binding density was always higher in dissected out myometrial vessels than in paired vessel-free myometrium (median 372 compared with 120 fmol\/mg protein; n = 20; P &amp;lt; 0.001). The specific binding in the paired samples was strongly correlated (r = 0.8904, P &amp;lt; 0.0001).<\/jats:p>\n               <jats:p>4. The specific binding of 125I-labelled angiotensin II in \u2018vessel-free\u2019 myometrium was higher in samples from the follicular phase than in samples from the luteal phase (median 144 compared with 37 fmol\/mg; P &amp;lt; 0.02). A similar trend was found in the vessels themselves, but this failed to reach statistical significance (459 compared with 225 fmol\/mg; P = 0.051).<\/jats:p>\n               <jats:p>5. It is suggested that the down-regulation of the angiotensin type 2 receptors in the luteal (secretory) phase is a preparation for pregnancy, removing an inhibitory factor to uterine growth and vascularization and allowing greater prostacyclin synthesis.<\/jats:p>","DOI":"10.1042\/cs0900499","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:38:46Z","timestamp":1438947526000},"page":"499-505","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Angiotensin Receptors in Myometrium and Myometrial Vessels from Uteri of Women during the Follicular and Luteal Phases of the Menstrual Cycle and in Late Pregnancy"],"prefix":"10.1042","volume":"90","author":[{"given":"C.","family":"Bing","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology, University Hospital, Nottingham, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. R.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, University Hospital, Nottingham, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. Broughton","family":"Pipkin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, University Hospital, Nottingham, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/90\/6\/499\/466088\/cs0900499.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/90\/6\/499\/466088\/cs0900499.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:53:35Z","timestamp":1638399215000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/90\/6\/499\/76530\/Angiotensin-Receptors-in-Myometrium-and-Myometrial"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1996,6,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0900499","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,6,1]]}}}