{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T20:36:59Z","timestamp":1682368619713},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,9,1]]},"abstract":"<jats:p>1. The nephron sites involved in the blunted natriuretic response to frusemide during sodium depletion were investigated using micropuncture techniques in anaesthetized rats.<\/jats:p>\n               <jats:p>2. Glomerular filtration rate was lower, and fractional sodium reabsorption in the proximal convoluted tubule higher, in sodium-depleted than in sodium-replete rats. Consequently, sodium delivery to the loop of Henle was reduced (by approximately 35%) in the sodium-depleted animals. Intravenous frusemide (2.5 mg h\u22121 kg\u22121; urinary water and electrolyte losses replaced) had no effect on glomerular filtration rate or proximal tubular sodium reabsorption in either group.<\/jats:p>\n               <jats:p>3. The inhibitory effect of intravenous frusemide on fractional sodium reabsorption in the nephron segments constituting the loop of Henle (measured by free-flow micropuncture) was attenuated during sodium depletion. However, when loops of Henle were microperfused at identical rates with artificial late proximal tubular fluid, no difference in the responses of sodium-depleted and sodium-replete rats to intraluminal frusemide (10\u22125 mol\/l) could be detected.<\/jats:p>\n               <jats:p>4. In sodium-replete animals, the increased load of sodium delivered from the loop of Henle during frusemide administration resulted in a lowering of fractional sodium reabsorption in the distal tubule. In contrast, in sodium-depleted rats given frusemide, fractional distal sodium reabsorption tended to increase, so that values in the two groups of frusemide-treated animals were markedly different (0.30 \u00b10.04 versus 0.51 \u00b10.03).<\/jats:p>\n               <jats:p>5. It is concluded that the blunted natriuretic response to frusemide during sodium depletion results from at least three factors: a reduced sodium delivery to the loop of Henle; a reduced inhibitory effect of frusemide on fractional sodium reabsorption in the loop of Henle, which may be a consequence of the reduced sodium load; and enhanced fractional reabsorption of sodium in the distal tubule, which partially buffers the diuretic-induced increase in sodium delivery from the loop.<\/jats:p>","DOI":"10.1042\/cs0910299","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:39:37Z","timestamp":1438947577000},"page":"299-305","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mechanism of the Impaired Natriuretic Response to Frusemide during Sodium Depletion: A Micropuncture Study in Rats"],"prefix":"10.1042","volume":"91","author":[{"given":"D. G.","family":"Shirley","sequence":"first","affiliation":[{"name":"Department of Physiology, Charing Cross and Westminster Medical School, London, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. J.","family":"Walter","sequence":"additional","affiliation":[{"name":"Department of Physiology, Charing Cross and Westminster Medical School, London, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Unwin","sequence":"additional","affiliation":[{"name":"Division of Nephrology, University College London Medical School, London, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/91\/3\/299\/466335\/cs0910299.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/91\/3\/299\/466335\/cs0910299.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T23:05:35Z","timestamp":1638399935000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/91\/3\/299\/76786\/Mechanism-of-the-Impaired-Natriuretic-Response-to"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0910299","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,9,1]]}}}