{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T01:14:36Z","timestamp":1697418876995},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,9,1]]},"abstract":"<jats:p>1. The K+\/Cl\u2212 co-transport system is activated by a number of interventions, such as cell swelling and stimulation with N-ethylmaleimide. It is specifically inhibited by R(+)-[(dihydroindenyl)oxy]alkanoic acid and requires the presence of K+ and Cl\u2212 on the same side of the cell membrane. This co-transporter has been studied extensively, mainly in erythrocytes of many species, in which it plays a key role in cell volume regulation. Here we present evidence that human platelets contain K+\/Cl\u2212 co-transporters.<\/jats:p>\n               <jats:p>2. We have studied the efflux of 86Rb+ (a marker for K+) from 86Rb+-loaded human platelets, and have defined their response to stimulation by N-ethylmaleimide.<\/jats:p>\n               <jats:p>3. N-Ethylmaleimide (0.5 and 1 mmol\/l) stimulated an increase in cumulative 86Rb+ efflux in a concentration-dependent manner. This efflux was inhibited by R(+)-[(dihydroindenyl)oxy]alkanoic acid (10 \u03bcmol\/l) but was insensitive to bumetanide. It also required the presence of external Cl\u2212.<\/jats:p>\n               <jats:p>4. These observations suggest that 86Rb+ efflux from the platelets stimulated by N-ethylmaleimide occurs via K+\/Cl\u2212 co-transport.<\/jats:p>\n               <jats:p>5. When the K+\/Cl\u2212 co-transporter was stimulated by N-ethylmaleimide we were unable to stimulate the Na+\/K+\/2Cl\u2212 co-transporter with a high external concentration of KCl or inhibit 86Rb+ efflux with bumetanide. Together with other evidence, this suggests that when the K+\/Cl\u2212 co-transporter is stimulated with N-ethylmaleimide, the Na+\/K+\/2Cl\u2212 co-transporter is inhibited.<\/jats:p>","DOI":"10.1042\/cs0930243","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:39:55Z","timestamp":1438947595000},"page":"243-248","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evidence for an R(+)-[(dihydroindenyl)oxy]alkanoic Acid-Sensitive K+\/Cl\u2212 co-Transporter in Human Platelets and its Interaction with the Na+\/K+\/2Cl\u2212 co-transporter"],"prefix":"10.1042","volume":"93","author":[{"given":"H. A.","family":"de Silva","sequence":"first","affiliation":[{"name":"University Department of Clinical Pharmacology, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. K.","family":"Aronson","sequence":"additional","affiliation":[{"name":"University Department of Clinical Pharmacology, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/93\/3\/243\/466575\/cs0930243.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/93\/3\/243\/466575\/cs0930243.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:59:49Z","timestamp":1638399589000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/93\/3\/243\/76741\/Evidence-for-an-R-dihydroindenyl-oxy-alkanoic-Acid"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0930243","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9,1]]}}}