{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T07:32:54Z","timestamp":1655969574521},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,10,1]]},"abstract":"<jats:p>1. Intracellular [Cl\u2212] ([Cl\u2212]i) was measured with ion-selective microelectrodes in rat femoral arterial smooth muscle in normotensive controls and after the induction of deoxycorticosterone acetate\/salt hypertension.<\/jats:p>\n               <jats:p>2. Linear regression of [Cl\u2212]i and time after the induction of hypertension showed good correlation (r = 0.96) for 5\u20136 weeks, as [Cl\u2212]i increased from 30 \u00b1 1 mmol\/l (mean \u00b1 SD, n = 16), to 49 \u00b1 2 mmol\/l (n = 9, P &amp;lt; 0.0001).<\/jats:p>\n               <jats:p>3. Arterial systolic blood pressure also increased linearly (r = 0.97) for 5\u20136 weeks as hypertension developed from 122 \u00b1 1 mmHg (n = 20) to 187 \u00b1 7 mmHg (n = 14): there was consequently a linear relationship between [Cl\u2212]i and arterial systolic blood pressure (r = 0.96).<\/jats:p>\n               <jats:p>4. The increase in [Cl\u2212]i was partly because Na+\u2212K+\u2212Cl\u2212 co-transport activity, estimated from the fall in [Cl\u2212]i caused by bumetanide, was greater in hypertension (18 mmol\/l) than in normotension (10 mmol\/l). This finding, and the depolarization of the membrane potential in hypertension (\u221256 \u00b1 3 mV compared with \u221264 \u00b1 4 mV in normotension; P &amp;lt; 0.0001), confirms previous studies.<\/jats:p>\n               <jats:p>5. The increase in [Cl\u2212]i was also partly due to greater activity of an Na+- and HCO3\u2212-independent, acetazolamide-sensitive inward Cl\u2212 transport system; thus acetazolamide reduced [Cl\u2212]i by 7 mmol\/l in normotension and by 16 mmol\/l in hypertension.<\/jats:p>\n               <jats:p>6. In Cl\u2212-free media, the membrane potential in normotension (\u221259 \u00b1 5 mV) was not significantly different from that in hypertension (\u221260 \u00b1 4 mV).<\/jats:p>\n               <jats:p>7. The role of [Cl\u2212]i in the depolarization of the membrane potential in hypertension is discussed.<\/jats:p>","DOI":"10.1042\/cs0930295","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:39:55Z","timestamp":1438947595000},"page":"295-298","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Activation of Two Inward Chloride Transport Systems in Rat Femoral Arterial Smooth Muscle in Deoxycorticosterone Acetate\/Salt Hypertension"],"prefix":"10.1042","volume":"93","author":[{"given":"Alexander A.","family":"Harper","sequence":"first","affiliation":[{"name":"Department of Anatomy and Physiology, University of Dundee, Dundee DD1 4HN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian P. L.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, University of Dundee, Dundee DD1 4HN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan R.","family":"Chipperfield","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, University of Dundee, Dundee DD1 4HN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/93\/4\/295\/466643\/cs0930295.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/93\/4\/295\/466643\/cs0930295.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T23:01:48Z","timestamp":1638399708000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/93\/4\/295\/76763\/Activation-of-Two-Inward-Chloride-Transport"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1997,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0930295","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,10,1]]}}}