{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T04:28:33Z","timestamp":1767155313772,"version":"build-2238731810"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:p>Previous studies have demonstrated a raised Na+ content in leucocytes isolated from women with pre-eclampsia. Increased Na+\/H+ exchanger activity is one membrane transport abnormality that may contribute to this phenomenon and may be implicated in the abnormal volume homoeostasis and hypertension associated with the disease. Increased Na+\/H+ exchanger activity has been documented in nucleated white blood cells from both pre-eclamptic and post-partum pre-eclamptic women, and may suggest the importance of genetic influences on exchanger activity. In the present study, we used lymphoblasts from women with pre-eclampsia and from age- and gestation-matched normotensive pregnant controls to determine Na+\/H+ exchanger activity and intracellular resting pH using fluorimetry and the pH-sensitive dye BCECF-AM [bis(carboxyethyl)carboxyfluorescein acetoxymethyl ester]. Determination of Na+\/H+ exchanger protein abundance was performed by Western blotting. Intracellular pH was not significantly different in cells from pre-eclamptic women compared with those from normotensive controls. Na+\/H+ exchanger activity was measured when the intracellular pH was clamped at 6.0, and was found to be significantly higher in cells from pre-eclamptic women (20.77\u00b10.92mmol\u00b7min-1\u00b7l-1) compared with those from normotensive controls (15.22\u00b10.92mmol\u00b7min-1\u00b7l-1; P = 0.001). Na+\/H+ exchanger protein abundance was established to be similar in the two subject groups, suggesting that the turnover number for the Na+\/H+ exchanger is increased in the women with pre-eclampsia. These changes in Na+\/H+ exchanger activity indicate the importance of genetic factors in determining this particular phenotype, since in this cell culture model of pre-eclampsia it is likely that environmental or hormonal influences present in vivo would have declined. Overactivity of the Na+\/H+ exchanger may contribute to the raised intracellular Na+ concentration reported previously in white blood cells from women with pre-eclampsia.<\/jats:p>","DOI":"10.1042\/cs1030503","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T07:43:51Z","timestamp":1438933431000},"page":"503-509","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Altered Na+\/H+ exchanger isoform 1 activity in immortalized lymphoblasts from women with pre-eclampsia: evidence for an intermediate phenotype"],"prefix":"10.1042","volume":"103","author":[{"given":"V.M.","family":"LEE","sequence":"first","affiliation":[{"name":"University of Leicester, Division of Medicine and Therapeutics, Level 4, Clinical Sciences Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.A.","family":"QUINN","sequence":"additional","affiliation":[{"name":"University of Leicester, Division of Medicine and Therapeutics, Level 4, Clinical Sciences Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.C.","family":"JENNINGS","sequence":"additional","affiliation":[{"name":"University of Leicester, Division of Medicine and Therapeutics, Level 4, Clinical Sciences Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.W.F.","family":"HALLIGAN","sequence":"additional","affiliation":[{"name":"University of Leicester, Division of Medicine and Therapeutics, Level 4, Clinical Sciences Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.L.","family":"NG","sequence":"additional","affiliation":[{"name":"University of Leicester, Division of Medicine and Therapeutics, Level 4, Clinical Sciences Building, Leicester Royal Infirmary, Leicester LE2 7LX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2002,10,23]]},"container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/103\/5\/503\/433483\/cs1030503.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/103\/5\/503\/433483\/cs1030503.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,14]],"date-time":"2023-05-14T20:17:49Z","timestamp":1684095469000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/103\/5\/503\/67338\/Altered-NaH-exchanger-isoform-1-activity-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,10,23]]},"references-count":0,"aliases":["10.1042\/cs20020098"],"journal-issue":{"issue":"5","published-print":{"date-parts":[[2002,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs1030503","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,10,23]]}}}