{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:05:52Z","timestamp":1774562752517,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"s2002","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,9,1]]},"abstract":"<jats:p>The receptors underlying the endothelin-dependent component of lung plasma extravasation and leucocyte infiltration induced by oleic acid were assessed in mice. Oleic acid (1mg\u00b7kg-1 intravenously), but not endothelin-1 (up to 1nmol\u00b7kg-1 intravenously), increased accumulation of Evans blue in the lungs (excluding the trachea and main bronchi) from 11.8\u00b13.9 to 98.6\u00b110.7\u00b5g 1h after injection. Bosentan, the antagonist of endothelin receptors (ETA and ETB) or the selective ETB receptor antagonists Ro 46-8443 or A-192621 (administered 1h before oleic acid at doses of 30, 10 and 30mg\u00b7kg-1 respectively) reduced the effect of oleic acid by 71%, 58% and 79% respectively. However, the selective ETA receptor antagonist A-127722.5 (10mg\u00b7kg-1) was inactive. Oleic acid (2mg\u00b7kg-1, intravenously) raised the number of total leucocytes, mononuclear cells and neutrophils in broncho-alveolar lavage fluid 4h after injection. Bosentan and Ro 46-8443 (at doses of 30 and 10mg\u00b7kg-1 respectively) inhibited the neutrophil infiltration induced by oleic acid by approx. 80%. None of the antagonists modified control (basal) pulmonary microvascular permeability or total and differential cell counts. Thus, endogenous endothelins, acting via ETB receptor-dependent mechanisms, play a major role in oleic acid-induced lung injury in the mouse by promoting infiltration of circulating neutrophils and enhancement of pulmonary microvascular plasma extravasation. These findings suggest that either ETB or mixed ETA\/ETB receptor antagonists might be beneficial in the treatment of the adult respiratory distress syndrome.<\/jats:p>","DOI":"10.1042\/cs103s340s","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:43:51Z","timestamp":1438947831000},"page":"340S-344S","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Endothelin ETB receptor-mediated mechanisms involved in oleic acid-induced acute lung injury in mice"],"prefix":"10.1042","volume":"103","author":[{"given":"Cl\u00e1udio L.","family":"GUIMAR\u00c3ES","sequence":"first","affiliation":[{"name":"Department of Pharmacology, CCB, Universidade Federal de Santa Catarina, Rua Ferreira Lima, 82, Florianopolis, 88015-420, SC, Brazil"},{"name":"Department of Natural Sciences, CCEN, Universidade Regional de Blumenau, Rua Antonio da Veiga, 140, Blumenau, 89010-971, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia G.","family":"TRENTIN","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, CCB, Universidade Federal de Santa Catarina, Rua Ferreira Lima, 82, Florianopolis, 88015-420, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giles A.","family":"RAE","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, CCB, Universidade Federal de Santa Catarina, Rua Ferreira Lima, 82, Florianopolis, 88015-420, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/103\/s2002\/340S\/440210\/cs103s340s.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/103\/s2002\/340S\/440210\/cs103s340s.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T22:20:19Z","timestamp":1638397219000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/103\/s2002\/340S\/69693\/Endothelin-ETB-receptor-mediated-mechanisms"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9,1]]},"references-count":0,"journal-issue":{"issue":"s2002","published-print":{"date-parts":[[2002,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs103s340s","relation":{},"ISSN":["0143-5221"],"issn-type":[{"value":"0143-5221","type":"print"}],"subject":[],"published":{"date-parts":[[2002,9,1]]}}}