{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T00:55:28Z","timestamp":1790988928160,"version":"4.1.0"},"reference-count":39,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,9,1]]},"abstract":"<jats:p>Systemic inflammatory response conditions are associated with capillary leak and haemodynamic compromise. Fluid resuscitation to reverse the ensuing hypovolaemia is, however, complicated by the decreased endothelium reflection coefficient to albumin and other colloids. We developed PEG\u2013Alb (albumin covalently linked to polyethylene glycol) as a potential resuscitative agent. PEG was covalently linked to human albumin at multiple sites on the protein. The modified protein was heterogeneous when examined by SDS\/PAGE, size-exclusion chromatography and SELDI\u2013TOF MS (surface-enhanced laser-desorption ionization\u2013time of flight MS). Based on size-exclusion chromatography and osmotic pressure data, the effective volume of PEG\u2013Alb is increased 13- to 16-fold compared with unmodified albumin. In an LPS (lipopolysaccharide) model of shock, rats treated with PEG\u2013Alb showed better blood pressure, lower Hct (haematocrit) consistent with haemodilution and less lung injury than rats treated with unmodified albumin or saline. In a CLP (caecal ligation and puncture) model of sepsis, PEG\u2013Alb was more effective than albumin or saline in maintaining blood pressure and in decreasing Hct. When fluorescein-labelled PEG\u2013Alb and Texas Red-labelled albumin were administered to rats with LPS- or CLP-induced shock, PEG\u2013Alb was retained within blood vessels, whereas albumin extravasates into the interstitial space. Based on these data, PEG\u2013Alb appears to be retained within blood vessels in models of capillary leak. PEG\u2013Alb may ultimately be effective in the clinical treatment of shock associated with capillary leak.<\/jats:p>","DOI":"10.1042\/cs20040001","type":"journal-article","created":{"date-parts":[[2004,8,25]],"date-time":"2004-08-25T14:11:29Z","timestamp":1093443089000},"page":"263-272","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Plasma expansion by polyethylene-glycol-modified albumin"],"prefix":"10.1042","volume":"107","author":[{"given":"Ragheb\u00a0A.","family":"ASSALY","sequence":"first","affiliation":[{"name":"Department of Medicine, Medical College of Ohio, 3120 Glendale Ave, Toledo, OH 43614, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"AZIZI","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Ohio, 3120 Glendale Ave, Toledo, OH 43614, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David\u00a0J.","family":"KENNEDY","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Ohio, 3120 Glendale Ave, Toledo, OH 43614, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristine","family":"AMAURO","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Ohio, 3120 Glendale Ave, Toledo, OH 43614, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiman","family":"ZAHER","sequence":"additional","affiliation":[{"name":"Department of Pathology, Medical College of Ohio, 3000 Arlington Ave, Toledo, OH 43614, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick\u00a0W.","family":"HOUTS","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Medical College of Ohio, 3035 Arlington Avenue, Toledo, OH 43614, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert\u00a0H.","family":"HABIB","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Ohio, 3120 Glendale Ave, Toledo, OH 43614, U.S.A."},{"name":"St Vincent Mercy Medical Center, 2213 Cherry St., Toledo, OH 43608, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph\u00a0I.","family":"SHAPIRO","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Ohio, 3120 Glendale Ave, Toledo, OH 43614, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u00a0David","family":"DIGNAM","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Medical College of Ohio, 3035 Arlington Avenue, Toledo, OH 43614, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2004,8,24]]},"reference":[{"key":"2021120120261229800_B1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1097\/00024382-199808000-00001","article-title":"Systemic inflammatory response syndrome (SIRS), multiple organ dysfunction syndrome (MODS), multiple organ failure (MOF): are we winning the battle?","volume":"10","author":"Baue","year":"1998","journal-title":"Shock"},{"key":"2021120120261229800_B2","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1007\/s000110050309","article-title":"Involvement and dual effects of nitric oxide in septic shock","volume":"47","author":"Wolkow","year":"1998","journal-title":"Inflamm. Res."},{"key":"2021120120261229800_B3","doi-asserted-by":"crossref","first-page":"621","DOI":"10.2165\/00003495-199855050-00002","article-title":"Colloid volume expanders. Problems, pitfalls and possibilities","volume":"55","author":"Roberts","year":"1998","journal-title":"Drugs"},{"key":"2021120120261229800_B4","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1136\/bmj.317.7153.240","article-title":"Why albumin may not work","volume":"317","author":"Berger","year":"1998","journal-title":"Br. Med. J."},{"key":"2021120120261229800_B5","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1177\/0148607191015002207","article-title":"Role of albumin in human physiology and pathophysiology","volume":"15","author":"Doweiko","year":"1991","journal-title":"JPEN, J. Parenter. Enteral Nutr."},{"key":"2021120120261229800_B6","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature (London)"},{"key":"2021120120261229800_B7","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1006\/abio.1996.0345","article-title":"Size-exclusion chromatography with on-line light-scattering, absorbance, and refractive index detectors for studying proteins and their interactions","volume":"240","author":"Wen","year":"1996","journal-title":"Anal. Biochem."},{"key":"2021120120261229800_B8","doi-asserted-by":"crossref","first-page":"364","DOI":"10.2144\/03342rr07","article-title":"Multifactorial screening design and analysis of SELDI-TOF ProteinChip array optimization experiments","volume":"34","author":"Cordingley","year":"2003","journal-title":"BioTechniques"},{"key":"2021120120261229800_B9","article-title":"Physical Chemistry of Macromolecules","author":"Tanford","year":"1961"},{"key":"2021120120261229800_B10","doi-asserted-by":"crossref","first-page":"C1543","DOI":"10.1152\/ajpcell.1997.272.5.C1543","article-title":"Induction of human endothelial cell apoptosis requires both heat shock and oxidative stress responses","volume":"272","author":"Wang","year":"1997","journal-title":"Am. J. Physiol."},{"key":"2021120120261229800_B11","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1183\/09031936.98.12040831","article-title":"Induction of nitric oxide synthase by lipopolysaccharide inhalation enhances substance P-induced microvascular leakage in guinea-pigs","volume":"12","author":"Ohuchi","year":"1998","journal-title":"Eur. Respir. J."},{"key":"2021120120261229800_B12","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1159\/000049698","article-title":"Cecal ligation and puncture as a model of sepsis in the rat: influence of the puncture size on mortality, bacteremia, endotoxemia and tumor necrosis factor \u03b1 levels","volume":"33","author":"Otero-Anton","year":"2001","journal-title":"Eur. Surg. Res."},{"key":"2021120120261229800_B13","first-page":"148","article-title":"Hemodynamic and metabolic alterations during experimental sepsis in young and adult rats","volume":"168","author":"Pedersen","year":"1989","journal-title":"Surg. Gynecol. Obstet."},{"key":"2021120120261229800_B14","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1023\/A:1027366403913","article-title":"The pulmonary inflammatory response to experimental fecal peritonitis: relative roles of tumor necrosis factor-\u03b1 and endotoxin","volume":"21","author":"Mercer-Jones","year":"1997","journal-title":"Inflammation"},{"key":"2021120120261229800_B15","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1097\/00024382-200013010-00004","article-title":"Cecal ligation and double puncture impairs heat shock protein 70 (HSP-70) expression in the lungs of rats","volume":"13","author":"Weiss","year":"2000","journal-title":"Shock"},{"key":"2021120120261229800_B16","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1002\/jms.508","article-title":"Evaluation of the quantitativeness of matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry using an equimolar mixture of uniform poly(ethylene glycol) oligomers","volume":"38","author":"Shimada","year":"2003","journal-title":"J. Mass. Spectrom."},{"key":"2021120120261229800_B17","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1002\/rcm.898","article-title":"Mass calibration of a matrix-assisted laser desorption\/ionization time-of-flight mass spectrometer including the rise time of the delayed extraction pulse","volume":"17","author":"Moskovets","year":"2003","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"2021120120261229800_B18","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1023\/A:1016113117851","article-title":"Preparation and characterization of mono-PEGylated epidermal growth factor: evaluation of in vitro biologic activity","volume":"19","author":"Lee","year":"2002","journal-title":"Pharm. Res."},{"key":"2021120120261229800_B19","doi-asserted-by":"crossref","first-page":"5189","DOI":"10.1021\/bi00264a013","article-title":"Small-angle X-ray scattering study of halophilic malate dehydrogenase","volume":"21","author":"Reich","year":"1982","journal-title":"Biochemistry"},{"key":"2021120120261229800_B20","first-page":"77","article-title":"The subdomain structure of human serum albumin in solution under different pH conditions studied by small angle X-ray scattering","volume":"24","author":"Olivieri","year":"1995","journal-title":"Eur. Biophys. J."},{"key":"2021120120261229800_B21","doi-asserted-by":"crossref","first-page":"S109","DOI":"10.1097\/00003246-200107001-00035","article-title":"Epidemiology of sepsis: an update","volume":"29","author":"Angus","year":"2001","journal-title":"Crit. Care Med."},{"key":"2021120120261229800_B22","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1097\/00003643-200310000-00003","article-title":"Albumin administration: what is the evidence of clinical benefit? A systematic review of randomized controlled trials","volume":"20","author":"Haynes","year":"2003","journal-title":"Eur. J. Anaesthesiol."},{"key":"2021120120261229800_B23","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1177\/0148607191015004484","article-title":"Use of albumin as a volume expander","volume":"15","author":"Doweiko","year":"1991","journal-title":"J. Parenter. Enteral. Nutr."},{"key":"2021120120261229800_B24","doi-asserted-by":"crossref","first-page":"370","DOI":"10.7326\/0003-4819-137-5_Part_1-200209030-00024","article-title":"Colloid use in the critically ill","volume":"137","author":"Wilkes","year":"2002","journal-title":"Ann. Intern. Med."},{"key":"2021120120261229800_B25","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1136\/bmj.317.7162.829","article-title":"Albumin: don't confuse us with the facts. Rather than fulminating, seek to answer the questions raised","volume":"317","author":"McClelland","year":"1998","journal-title":"Br. Med. J."},{"key":"2021120120261229800_B26","doi-asserted-by":"crossref","first-page":"149","DOI":"10.7326\/0003-4819-135-3-200108070-00007","article-title":"Patient survival after human albumin administration. A meta-analysis of randomized, controlled trials","volume":"135","author":"Wilkes","year":"2001","journal-title":"Ann. Intern. Med."},{"key":"2021120120261229800_B27","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1136\/bmj.317.7153.235","article-title":"Human albumin administration in critically ill patients: systematic review of randomised controlled trials","volume":"317","author":"Cochrane","year":"1998","journal-title":"Br. Med. J."},{"key":"2021120120261229800_B28","first-page":"2440","article-title":"Equivalent pore modeling: vesicles and channels","volume":"42","author":"Taylor","year":"1983","journal-title":"Fed. Proc."},{"key":"2021120120261229800_B29","first-page":"169","article-title":"Increased microvascular permeability in canine endotoxic shock: protective effects of ibuprofen","volume":"26","author":"Hubbard","year":"1988","journal-title":"Circ. Shock"},{"key":"2021120120261229800_B30","first-page":"249","article-title":"The uses and properties of PEG-linked proteins","volume":"9","author":"Delgado","year":"1992","journal-title":"Crit. Rev. Ther. Drug Carrier Syst."},{"key":"2021120120261229800_B31","doi-asserted-by":"crossref","first-page":"3578","DOI":"10.1016\/S0021-9258(17)40291-2","article-title":"Alteration of immunological properties of bovine serum albumin by covalent attachment of polyethylene glycol","volume":"252","author":"Abuchowski","year":"1977","journal-title":"J. Biol. Chem."},{"key":"2021120120261229800_B32","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1111\/j.1365-2141.1992.tb06940.x","article-title":"Polyethylene glycol (PEG) modification of granulocyte-macrophage colony stimulating factor (GM-CSF) enhances neutrophil priming activity but not colony stimulating activity","volume":"82","author":"Knusli","year":"1992","journal-title":"Br. J. Haematol."},{"key":"2021120120261229800_B33","first-page":"238","article-title":"The effects of hemodilution with polyethylene glycol bovine hemoglobin (PEG-Hb) in a conscious porcine model","volume":"44","author":"Conover","year":"1996","journal-title":"J. Invest. Med."},{"key":"2021120120261229800_B34","first-page":"F446","article-title":"Pathogenetic mechanisms in experimental hemoglobinuric acute renal failure","volume":"256","author":"Zager","year":"1989","journal-title":"Am. J. Physiol."},{"key":"2021120120261229800_B35","first-page":"87","article-title":"Pegylated interferons","volume":"1","author":"Baker","year":"2001","journal-title":"Rev. Gastroenterol. Disord."},{"key":"2021120120261229800_B36","first-page":"1059","article-title":"Improved pharmacokinetic properties of a polyethylene glycol-modified form of interferon-\u03b2-1a with preserved in vitro bioactivity","volume":"297","author":"Pepinsky","year":"2001","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"2021120120261229800_B37","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1248\/cpb.48.1921","article-title":"Pharmacokinetic disposition of polyethylene glycol-modified salmon calcitonins in rats","volume":"48","author":"Yoo","year":"2000","journal-title":"Chem. Pharm. Bull. (Tokyo)"},{"key":"2021120120261229800_B38","doi-asserted-by":"crossref","first-page":"2478","DOI":"10.1046\/j.1523-1755.2000.00431.x","article-title":"Pharmacodynamics and pharmacokinetics of polyethylene glycol-hirudin in patients with chronic renal failure","volume":"58","author":"Poschel","year":"2000","journal-title":"Kidney Int."},{"key":"2021120120261229800_B39","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1006\/phrs.1993.1117","article-title":"Pharmacokinetics and distribution of ribonuclease and its monomethoxypoly(ethylene glycol) derivatives in rats","volume":"28","author":"Laznicek","year":"1993","journal-title":"Pharmacol. Res."}],"container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/107\/3\/263\/436100\/cs1070263.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/107\/3\/263\/436100\/cs1070263.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T20:30:00Z","timestamp":1638390600000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/107\/3\/263\/67679\/Plasma-expansion-by-polyethylene-glycol-modified"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8,24]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs20040001","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,8,24]]}}}