{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T23:26:53Z","timestamp":1771025213840,"version":"3.50.1"},"reference-count":42,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,9,1]]},"abstract":"<jats:p>The aim of the present study was to investigate the effects of (i) the pro-inflammatory cytokines IL (interleukin)-1\u03b2, TNF-\u03b1 (tumour necrosis factor-\u03b1), IFN-\u03b3 (interferon-\u03b3) and anti-inflammatory cytokines IL-4 and IL-13, and (ii) NO (nitric oxide) donors on HA (hyaluronic acid) production by synovial cells from patients with rheumatoid arthritis. Synovial cells obtained from five patients with rheumatoid arthritis were incubated for 24\u00a0h without or with IL-1\u03b2, TNF-\u03b1, IFN-\u03b3, or with this mixture for 24\u00a0h plus IL-4 or IL-13 for the last 6\u00a0h. The same cells were also incubated for 3\u201324\u00a0h without or with SNP (sodium nitroprusside) or SNAP (S-nitroso-N-acetyl-DL-penicillamine). HA secretion was determined by an immunoenzymic assay based on HA-specific binding by proteoglycan isolated from bovine cartilage. IL-1\u03b2, TNF-\u03b1 and IFN-\u03b3 alone or in combination stimulated HA synthesis, whereas IL-4 and IL-13 dose-dependently inhibited HA production induced by Th1 cytokines. HA production was significantly increased by the presence of 1\u00a0mM SNP after 6 and 12\u00a0h (maximal effect). HA production was significantly increased by the presence of 0.01 and 0.1\u00a0mM SNAP after 12\u00a0h of incubation, and cells treated with 1\u00a0mM SNAP showed a maximal HA production after 24\u00a0h of incubation. In conclusion, the present study provides data concerning the regulatory role of pro- and anti-inflammatory cytokines and NO donors on HA metabolism in rheumatoid synovial cells and may help in understanding the pathophysiology of rheumatoid arthritis.<\/jats:p>","DOI":"10.1042\/cs20040104","type":"journal-article","created":{"date-parts":[[2004,8,25]],"date-time":"2004-08-25T14:11:29Z","timestamp":1093443089000},"page":"291-296","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Effects of pro- and anti-inflammatory cytokines and nitric oxide donors on hyaluronic acid synthesis by synovial cells from patients with rheumatoid arthritis"],"prefix":"10.1042","volume":"107","author":[{"given":"Camille","family":"CHENEVIER-GOBEAUX","sequence":"first","affiliation":[{"name":"Department of Biochemistry, H\u00f4pital Cochin, Assistance Publique \u2013 H\u00f4pitaux de Paris, 27 rue du Faubourg Saint-Jacques, 75679 Paris cedex 14, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9verine","family":"MORIN-ROBINET","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, H\u00f4pital Cochin, Assistance Publique \u2013 H\u00f4pitaux de Paris, 27 rue du Faubourg Saint-Jacques, 75679 Paris cedex 14, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herv\u00e9","family":"LEMARECHAL","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, H\u00f4pital Cochin, Assistance Publique \u2013 H\u00f4pitaux de Paris, 27 rue du Faubourg Saint-Jacques, 75679 Paris cedex 14, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serge","family":"POIRAUDEAU","sequence":"additional","affiliation":[{"name":"Department of Physical and Rehabilitation Medicine, H\u00f4pital Cochin, Assistance Publique \u2013 H\u00f4pitaux de Paris, 27 rue du Faubourg Saint-Jacques, 75679 Paris cedex 14, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Charles\u00a0O.\u00a0G.","family":"EKINDJIAN","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, H\u00f4pital Cochin, Assistance Publique \u2013 H\u00f4pitaux de Paris, 27 rue du Faubourg Saint-Jacques, 75679 Paris cedex 14, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Didier","family":"BORDERIE","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, H\u00f4pital Cochin, Assistance Publique \u2013 H\u00f4pitaux de Paris, 27 rue du Faubourg Saint-Jacques, 75679 Paris cedex 14, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2004,8,24]]},"reference":[{"key":"2021120120252056200_B1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1186\/ar113","article-title":"Fibroblast biology: role of synovial fibroblasts in the pathogenesis of rheumatoid arthritis","volume":"2","author":"Pap","year":"2000","journal-title":"Arthritis Res."},{"key":"2021120120252056200_B2","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1016\/S0140-6736(01)06075-5","article-title":"Rheumatoid arthritis","volume":"358","author":"Lee","year":"2001","journal-title":"Lancet"},{"key":"2021120120252056200_B3","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.4049\/jimmunol.152.4.2060","article-title":"Production of interleukin-1 receptor antagonist, inflammatory chemotactic proteins, and prostaglandin E by rheumatoid and osteoarthritic synoviocytes: regulation by IFN-\u03b3 and IL-4","volume":"152","author":"Seitz","year":"1994","journal-title":"J. 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