{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T15:32:53Z","timestamp":1767713573841},"reference-count":32,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,5,1]]},"abstract":"<jats:p>Novel therapies for the treatment of MOF (multiple organ failure) are required. In the present study, we examined the effect of synthetic GHRP-6 (growth hormone-releasing peptide-6) on cell migration and proliferation using rat intestinal epithelial (IEC-6) and human colonic cancer (HT29) cells as in vitro models of injury. In addition, we examined its efficacy when given alone and in combination with the potent protective factor EGF (epidermal growth factor) in an in vivo model of MOF (using two hepatic vessel ischaemia\/reperfusion protocols; 45\u00a0min of ischaemia and 45\u00a0min of reperfusion or 90\u00a0min of ischaemia and 120\u00a0min of reperfusion). In vitro studies showed that GHRP-6 directly influenced gut epithelial function as its addition caused a 3-fold increase in the rate of cell migration of IEC-6 and HT29 cells (P&amp;lt;0.01), but did not increase proliferation ([3H]thymidine incorporation). In vivo studies showed that, compared with baseline values, ischaemia\/reperfusion caused marked hepatic and intestinal damage (histological scoring), neutrophilic infiltration (myeloperoxidase assay; 5-fold increase) and lipid peroxidation (malondialdehyde assay; 4-fold increase). Pre-treatment with GHRP-6 (120\u00a0\u03bcg\/kg of body weight, intraperitoneally) alone truncated these effects by 50\u201385% (all P&amp;lt;0.05) and an additional benefit was seen when GHRP-6 was used in combination with EGF (1\u00a0mg\/kg of body weight, intraperitoneally). Lung and renal injuries were also reduced by these pre-treatments. In conclusion, administration of GHRP-6, given alone or in combination with EGF to enhance its effects, may provide a novel simple approach for the prevention and treatment of MOF and other injuries of the gastrointestinal tract. In view of these findings, further studies appear justified.<\/jats:p>","DOI":"10.1042\/cs20050374","type":"journal-article","created":{"date-parts":[[2006,4,11]],"date-time":"2006-04-11T16:45:57Z","timestamp":1144773957000},"page":"563-573","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Use of growth-hormone-releasing peptide-6 (GHRP-6) for the prevention of multiple organ failure"],"prefix":"10.1042","volume":"110","author":[{"given":"Danay","family":"Cibri\u00e1n","sequence":"first","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Ave 31 e\/158 & 190 Playa 10600, Havana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hussam","family":"Ajamieh","sequence":"additional","affiliation":[{"name":"Center for Biological Studies, Food and Drug Institute, University of Havana, Ave 23 e\/44 & 222 La Coronela, La Lisa, Havana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Berlanga","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Ave 31 e\/158 & 190 Playa 10600, Havana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga\u00a0S.","family":"Le\u00f3n","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Ave 31 e\/158 & 190 Playa 10600, Havana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose\u00a0S.","family":"Alba","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Ave 31 e\/158 & 190 Playa 10600, Havana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micheal\u00a0J.-T.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tania","family":"Marchbank","sequence":"additional","affiliation":[{"name":"Imperial College School of Medicine, Barts & The London, Queen Mary's School of Medicine and Dentistry, Turner Street, London E1 2AD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph\u00a0J.","family":"Boyle","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Freya","family":"Freyre","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Ave 31 e\/158 & 190 Playa 10600, Havana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Garcia\u00a0Del\u00a0Barco","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Ave 31 e\/158 & 190 Playa 10600, Havana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Lopez-Saura","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Ave 31 e\/158 & 190 Playa 10600, Havana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerardo","family":"Guillen","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Ave 31 e\/158 & 190 Playa 10600, Havana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subrata","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert\u00a0A.","family":"Goodlad","sequence":"additional","affiliation":[{"name":"Cancer Research UK, Lincoln's Inn Fields, London WC2A 3PX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond\u00a0J.","family":"Playford","sequence":"additional","affiliation":[{"name":"Imperial College School of Medicine, Barts & The London, Queen Mary's School of Medicine and Dentistry, Turner Street, London E1 2AD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2006,4,11]]},"reference":[{"key":"2021120120424209200_B1","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s002689900063","article-title":"Severity stratification and outcome prediction for multisystem organ failure and dysfunction","volume":"20","author":"Zimmerman","year":"1996","journal-title":"World J. 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