{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T18:53:37Z","timestamp":1762541617930},"reference-count":37,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,2,1]]},"abstract":"<jats:p>In the present study, we have analysed the mechanisms of Ca2+ entry and release in platelets obtained from BDL (bile-duct-ligated) rats, 11\u201313\u00a0days and 4\u00a0weeks after surgery. Platelets were washed and loaded with fura-2, and [Ca2+]i (cytosolic Ca2+ concentration) was determined in cell suspensions by means of fluorescence spectroscopy. Basal [Ca2+]i was similar in platelets from BDL rats compared with those from their respective controls, both in the absence and presence of extracellular Ca2+. Platelet stimulation with thrombin in the absence and presence of extracellular Ca2+ induced a rapid rise in [Ca2+]i that was of greater magnitude in platelets from BDL rats than in controls. Ca2+ storage was significantly elevated in platelets from BDL rats, as well as the activity of SERCA (sarcoplasmic\/endoplasmic-reticulum Ca2+-ATPase). Capacitative Ca2+ entry, as evaluated by inhibition of SERCA with thapsigargin, was also altered in platelets from BDL rats, having lower rates of Ca2+ entry. In conclusion, chronic BDL alters intracellular Ca2+ homoeostasis in platelets, such that an enhanced Ca2+ release is evoked by thrombin, which may be due to an increased amount of Ca2+ stored in the intracellular organelles and secondary to an enhanced activity of SERCA. These alterations are already evident before cirrhosis has completely developed and occurs during the cholestasis phase.<\/jats:p>","DOI":"10.1042\/cs20060226","type":"journal-article","created":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T09:38:01Z","timestamp":1173951481000},"page":"167-174","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Altered calcium signalling in platelets from bile-duct-ligated rats"],"prefix":"10.1042","volume":"112","author":[{"given":"Noem\u00ed\u00a0M.","family":"Atucha","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Iy\u00fa","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonia","family":"Alcaraz","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Rosa","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Concepci\u00f3n","family":"Mart\u00ednez-Prieto","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.\u00a0Clara","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan\u00a0Antonio","family":"Rosado","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Extremadura, C\u00e1ceres 10071, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaqu\u00edn","family":"Garc\u00eda-Esta\u00f1","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2007,1,3]]},"reference":[{"key":"2021120120411322000_B1","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1016\/0016-5085(89)90526-X","article-title":"Prospective evaluation of esophageal varices in primary biliary cirrhosis: development, natural history, and influence on survival","volume":"96","author":"Gores","year":"1989","journal-title":"Gastroenterology"},{"key":"2021120120411322000_B2","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1093\/bja\/69.3.307","article-title":"Thrombelastography","volume":"69","author":"Mallett","year":"1992","journal-title":"Br. 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