{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:09:32Z","timestamp":1787004572184,"version":"build-2736575974"},"reference-count":48,"publisher":"Portland Press Ltd.","issue":"9","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,5,1]]},"abstract":"<jats:p>Coronary, vascular and perivascular inflammation in rats following MR (mineralocorticoid receptor) activation plus salt are well-characterized precursors for the appearance of cardiac fibrosis. Endogenous corticosterone, in the presence of the 11\u03b2HSD2 (11\u03b2 hydroxysteroid dehydrogenase type\u00a02) inhibitor CBX (carbenoxolone) plus salt, produces similar inflammatory responses and tissue remodelling via activation of MR. MR-mediated oxidative stress has previously been suggested to account for these responses. In the present study we thus postulated that when 11\u03b2HSD2 is inhibited, endogenous corticosterone bound to unprotected MR in the vessel wall may similarly increase early biomarkers of oxidative stress. Uninephrectomized rats received either DOC (deoxycorticosterone), CBX or CBX plus the MR antagonist EPL (eplerenone) together with 0.9% saline to drink for 4, 8 or 16\u00a0days. Uninephrectomized rats maintained on 0.9% saline for 8\u00a0days served as controls. After 4\u00a0days, both DOC and CBX increased both macrophage infiltration and mRNA expression of the p22phox subunit of NADPH oxidase, whereas CBX, but not DOC, increased expression of the NOX2 (gp91phox) subunit. eNOS [endothelial NOS (NO synthase)] mRNA expression significantly decreased from 4\u00a0days for both treatments, and iNOS (inducible NOS) mRNA levels increased after 16\u00a0days of DOC or CBX; co-administration of EPL inhibited all responses to CBX. The responses characterized over this time course occurred before measurable increases in cardiac hypertrophy or fibrosis. The findings of the present study support the hypothesis that endogenous corticosterone in the presence of CBX can activate vascular MR to produce both inflammatory and oxidative tissue responses well before the onset of fibrosis, that the two MR ligands induce differential but overlapping patterns of gene expression, and that elevation of NOX2 subunit levels does not appear necessary for full expression of MR-mediated inflammatory and fibrogenic responses.<\/jats:p>","DOI":"10.1042\/cs20080247","type":"journal-article","created":{"date-parts":[[2008,11,6]],"date-time":"2008-11-06T10:37:28Z","timestamp":1225967848000},"page":"731-739","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Mediators of mineralocorticoid receptor-induced profibrotic inflammatory responses in the heart"],"prefix":"10.1042","volume":"116","author":[{"given":"Peter","family":"Wilson","sequence":"first","affiliation":[{"name":"Prince Henry's Institute of Medical Research, P.O. 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