{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:02:57Z","timestamp":1761580977505,"version":"3.40.3"},"reference-count":54,"publisher":"Portland Press Ltd.","issue":"7","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,10,1]]},"abstract":"<jats:p>Sympathetic activities are elevated in the central SNSs (sympathetic nervous systems) of hypertensive animals, but it is not known whether sympathetic innervation is also elevated in the heart. Sympathetic hyper-responsiveness in hypertension may result from oxidative stress. The aim of the present study was to investigate sympathetic hyperinnervation in DOCA (deoxycorticosterone acetate)-salt hypertensive rats with established hypertension. At 4\u00a0weeks after the start of DOCA-salt treatment and uninephrectomization, male Wistar rats were randomized into three groups for 8 weeks: vehicle, NAC (N-acetylcysteine) and triple therapy (hydralazine, hydrochlorothiazide and reserpine). DOCA-salt was associated with increased oxidant release. DOCA-salt produced concentric left ventricular hypertrophy and cardiomyocyte hypertrophy. Sympathetic hyperinnervation was observed in DOCA-salt rats, as assessed by myocardial noradrenaline levels, immunofluorescent analysis of tyrosine hydroxylase, growth-associated factor 43 and neurofilament and Western blotting and real-time quantitative RT\u2013PCR (reverse transcription\u2013PCR) of NGF (nerve growth factor). Arrhythmic scores during programmed stimulation in DOCA-salt rats were significantly higher than those in the control rats. Triple therapy, despite being effective on BP (blood pressure), offered neither attenuated cardiomyocyte hypertrophy nor anti-arrhythmia. The effects of DOCA-salt treatment on NGF expression, sympathetic hyperinnervation and arrhythmias were attenuated by NAC. Furthermore, the effects of NAC on NGF were abolished by administering BSO (L-buthionine sulfoximine), an inhibitor of glutamate\u2013cysteine ligase. In conclusion, DOCA-salt treatment contributes to up-regulation of NGF proteins probably through a free radical-dependent pathway in a BP-independent manner. DOCA-salt rats treated with NAC attenuate sympathetic hyperinnervation and thus show a beneficial effect on arrhythmogenic response to programmed electrical stimulation.<\/jats:p>","DOI":"10.1042\/cs20120080","type":"journal-article","created":{"date-parts":[[2012,4,18]],"date-time":"2012-04-18T09:58:10Z","timestamp":1334743090000},"page":"445-457","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cardiac sympathetic hyperinnervation in deoxycorticosterone acetate-salt hypertensive rats"],"prefix":"10.1042","volume":"123","author":[{"given":"Tsung-Ming","family":"Lee","sequence":"first","affiliation":[{"name":"Cardiology Section, Department of Medicine, Chi-Mei Medical Center, Tainan, Taiwan"},{"name":"Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien-Chang","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, National Cheng-Kung University, Tainan, Taiwan"},{"name":"Division of Cardiovascular Surgery, Chia-yi Christian Hospital, Chia-yi City, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nen-Chung","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan"},{"name":"Division of Cardiology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan"},{"name":"Cardiovascular Research Center, Taipei Medical University Hospital, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2012,6,7]]},"reference":[{"key":"2021113010255068800_B1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1097\/00004872-200102000-00002","article-title":"Arterial hypertension and cardiac arrhythmias","volume":"19","author":"Hennersdorf","year":"2001","journal-title":"J. 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